@article{fdi:010065491,
  title = {{RNA}-seq de novo assembly reveals differential gene expression in {G}lossina palpalis gambiensis infected with {T}rypanosoma brucei gambiense vs. non-infected and self-cured flies},
  author = {{S}oumana, {I}. {H}. and {K}lopp, {C}. and {R}avel, {S}ophie and {N}abihoudine, {I}. and {T}chicaya, {B}. and {P}arrinello, {H}. and {A}bate, {L}uc and {R}ialle, {S}. and {G}eiger, {A}nne},
  editor = {},
  language = {{ENG}},
  abstract = {{T}rypanosoma brucei gambiense ({T}bg), causing the sleeping sickness chronic form, completes its developmental cycle within the tsetse fly vector {G}lossina palpalis gambiensis ({G}pg) before its transmission to humans. {W}ithin the framework of an anti vector disease control strategy, a global gene expression profiling of trypanosome infected (susceptible), non-infected, and self-cured (refractory) tsetse flies was performed, on their midguts, to determine differential genes expression resulting from in vivo trypanosomes, tsetse flies (and their microbiome) interactions. {A}n {RNA}seg de novo assembly was achieved. {T}he assembled transcripts were mapped to reference sequences for functional annotation. {T}wenty-four percent of the 16,936 contigs could not be annotated, possibly representing untranslated m{RNA} regions, or {G}pg- or {T}bg-specific {ORF}s. {T}he remaining contigs were classified into 65 functional groups. {O}nly a few transposable elements were present in the {G}pg midgut transcriptome, which may represent active transpositions and play regulatory roles. {O}ne thousand three hundred and seventy three genes differentially expressed ({DEG}s) between stimulated and non-stimulated flies were identified at day-3 post-feeding; 52 and 1025 between infected and self cured flies at 10 and 20 days post feeding, respectively. {T}he possible roles of several {DEG}s regarding fly susceptibility and refractoriness are discussed. {T}he results provide new means to decipher fly infection mechanisms, crucial to develop anti-vector control strategies.},
  keywords = {de nova assembly ; {G}lossina palpalis gambiensis ; human {A}frican trypanosomiasis ; in vivo metatranscriptomics ; {RNA}-seq ; {T}rypanosome brucei gambiense ; {AFRIQUE} {SUBSAHARIENNE}},
  booktitle = {},
  journal = {{F}rontiers in {M}icrobiology},
  volume = {6},
  numero = {},
  pages = {art. 1259 [19 p.]},
  ISSN = {1664-302{X}},
  year = {2015},
  DOI = {10.3389/fmicb.2015.01259},
  URL = {https://www.documentation.ird.fr/hor/fdi:010065491},
}