Angel-Cuapio A., Figueroa-Montero A., Favela-Torres E., Viniegra-Gonzalez G., Perraud Gaime Isabelle, Loera O. (2015). Critical values of porosity in rice cultures of Isaria fumosorosea by adding water hyacinth : effect on conidial yields and quality. Applied Biochemistry and Biotechnology, 177 (2), p. 446-457. ISSN 0273-2289.
Titre du document
Critical values of porosity in rice cultures of Isaria fumosorosea by adding water hyacinth : effect on conidial yields and quality
Angel-Cuapio A., Figueroa-Montero A., Favela-Torres E., Viniegra-Gonzalez G., Perraud Gaime Isabelle, Loera O.
Source
Applied Biochemistry and Biotechnology, 2015,
177 (2), p. 446-457 ISSN 0273-2289
Conidia of the entomopathogenic fungus Isaria fumosorosea are used to control insect pests in crops. Commercially available mycoinsecticides manufactured with this fungus are produced on a large scale via solid-state cultures (SSC). In order to favour gaseous exchange in SCC, texturizers can be added to increase porosity fraction (epsilon). This work presents results of water hyacinth (Eichhornia crassipes) as a novel texturizer. A mixture of parboiled rice (PR), with a epsilon = 0.23, was used as a substrate, which was then mixed with water hyacinth (WH amendment) as a texturizer at different proportions affecting epsilon. Strains CNRCB1 and ARSEF3302 of I. fumosorosea yielded 1.6 (1.49-1.71) x 10(9) and 7.3 (7.02-7.58) x 10(9) conidia per gram of initial dry rice after 8 days, at epsilon values of 0.34 and 0.36, respectively. Improvement of conidial yields corresponded to 1.33 and 1.55 times, respectively, compared to rice alone using WH amendment in the mixtures PR:WH (%) at 90-10 and 80-20. In addition, infectivity against Galleria mellonella larvae was maintained. This is the first report of the use of water hyacinth as a texturizer in SSC, affecting epsilon, which is proposed a key parameter in conidia production by I. fumosorosea, without affecting conidial infectivity.
