%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Angel-Cuapio, A.
%A Figueroa-Montero, A.
%A Favela-Torres, E.
%A Viniegra-Gonzalez, G.
%A Perraud Gaime, Isabelle
%A Loera, O.
%T Critical values of porosity in rice cultures of Isaria fumosorosea by adding water hyacinth : effect on conidial yields and quality
%D 2015
%L fdi:010064956
%G ENG
%J Applied Biochemistry and Biotechnology
%@ 0273-2289
%K Isaria fumosorosea ; Porosity ; Texturizer ; Solid-state culture (SSC) ; Infectivity
%M ISI:000360076200011
%N 2
%P 446-457
%R 10.1007/s12010-015-1754-4
%U https://www.documentation.ird.fr/hor/fdi:010064956
%> https://www.documentation.ird.fr/intranet/publi/2015/09/010064956.pdf
%V 177
%W Horizon (IRD)
%X Conidia of the entomopathogenic fungus Isaria fumosorosea are used to control insect pests in crops. Commercially available mycoinsecticides manufactured with this fungus are produced on a large scale via solid-state cultures (SSC). In order to favour gaseous exchange in SCC, texturizers can be added to increase porosity fraction (epsilon). This work presents results of water hyacinth (Eichhornia crassipes) as a novel texturizer. A mixture of parboiled rice (PR), with a epsilon = 0.23, was used as a substrate, which was then mixed with water hyacinth (WH amendment) as a texturizer at different proportions affecting epsilon. Strains CNRCB1 and ARSEF3302 of I. fumosorosea yielded 1.6 (1.49-1.71) x 10(9) and 7.3 (7.02-7.58) x 10(9) conidia per gram of initial dry rice after 8 days, at epsilon values of 0.34 and 0.36, respectively. Improvement of conidial yields corresponded to 1.33 and 1.55 times, respectively, compared to rice alone using WH amendment in the mixtures PR:WH (%) at 90-10 and 80-20. In addition, infectivity against Galleria mellonella larvae was maintained. This is the first report of the use of water hyacinth as a texturizer in SSC, affecting epsilon, which is proposed a key parameter in conidia production by I. fumosorosea, without affecting conidial infectivity.
%$ 084