Brengues Cécile, Ferre J. B., Le Goff Gilbert, Lami P., Pratlong F., Pasteur N., Lagneau C., Simard Frédéric, Robert Vincent. (2014). A multiplex PCR to differentiate the two sibling species of mosquitoes Ochlerotatus detritus and Oc. coluzzii and evidence for further genetic heterogeneity within the Detritus complex. Infection Genetics and Evolution, 28, p. 676-680. ISSN 1567-1348.
Titre du document
A multiplex PCR to differentiate the two sibling species of mosquitoes Ochlerotatus detritus and Oc. coluzzii and evidence for further genetic heterogeneity within the Detritus complex
Brengues Cécile, Ferre J. B., Le Goff Gilbert, Lami P., Pratlong F., Pasteur N., Lagneau C., Simard Frédéric, Robert Vincent
Source
Infection Genetics and Evolution, 2014,
28, p. 676-680 ISSN 1567-1348
Internal transcribed spacer regions of ribosomal DNA were sequenced, and species-specific primers were designed to simplify the identification of two morphologically similar species of the Detritus complex, Ochlerotatus detritus and Ochlerotatus coluzzii. Each newly designed primer was able to amplify a species-specific fragment with a different size. Samples from France and Germany were successfully tested. This new tool prompts for bio-ecological studies to refine basic knowledge on the bionomics of this species complex, towards a better control and prevention of ensuing nuisances. Moreover, ITS2 sequencing revealed the existence of (1) two distinct haplotypes of Oc. detritus that are sympatric and widely distributed along the French Atlantic and Mediterranean littorals and (2) a specific haplotype in mosquitoes sampled from Tunisia, raising the question of the taxonomic status of this North-African population.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
EUROPE ; FRANCE ; ALLEMAGNE ; AFRIQUE DU NORD ; TUNISIE
