@article{fdi:010063623,
  title = {{A} multiplex {PCR} to differentiate the two sibling species of mosquitoes {O}chlerotatus detritus and {O}c. coluzzii and evidence for further genetic heterogeneity within the {D}etritus complex},
  author = {{B}rengues, {C}{\'e}cile and {F}erre, {J}. {B}. and {L}e {G}off, {G}ilbert and {L}ami, {P}. and {P}ratlong, {F}. and {P}asteur, {N}. and {L}agneau, {C}. and {S}imard, {F}r{\'e}d{\'e}ric and {R}obert, {V}incent},
  editor = {},
  language = {{ENG}},
  abstract = {{I}nternal transcribed spacer regions of ribosomal {DNA} were sequenced, and species-specific primers were designed to simplify the identification of two morphologically similar species of the {D}etritus complex, {O}chlerotatus detritus and {O}chlerotatus coluzzii. {E}ach newly designed primer was able to amplify a species-specific fragment with a different size. {S}amples from {F}rance and {G}ermany were successfully tested. {T}his new tool prompts for bio-ecological studies to refine basic knowledge on the bionomics of this species complex, towards a better control and prevention of ensuing nuisances. {M}oreover, {ITS}2 sequencing revealed the existence of (1) two distinct haplotypes of {O}c. detritus that are sympatric and widely distributed along the {F}rench {A}tlantic and {M}editerranean littorals and (2) a specific haplotype in mosquitoes sampled from {T}unisia, raising the question of the taxonomic status of this {N}orth-{A}frican population.},
  keywords = {{M}osquito ; {A}edini ; {S}pecies determination ; {M}ultiplex {PCR} ; {EUROPE} ; {FRANCE} ; {ALLEMAGNE} ; {AFRIQUE} {DU} {NORD} ; {TUNISIE}},
  booktitle = {},
  journal = {{I}nfection {G}enetics and {E}volution},
  volume = {28},
  numero = {},
  pages = {676--680},
  ISSN = {1567-1348},
  year = {2014},
  DOI = {10.1016/j.meegid.2014.07.010},
  URL = {https://www.documentation.ird.fr/hor/fdi:010063623},
}