Publications des scientifiques de l'IRD

Bernard E., Hamel Rodolphe, Neyret A., Ekchariyawat P., Molès J.P., Simmons G., Chazal N., Desprès P., Missé Dorothée, Briant L. (2015). Human keratinocytes restrict chikungunya virus replication at a post-fusion step. Virology, 476, p. 1-10. ISSN 0042-6822.

Titre du document
Human keratinocytes restrict chikungunya virus replication at a post-fusion step
Année de publication
2015
Type de document
Article référencé dans le Web of Science WOS:000349883000001
Auteurs
Bernard E., Hamel Rodolphe, Neyret A., Ekchariyawat P., Molès J.P., Simmons G., Chazal N., Desprès P., Missé Dorothée, Briant L.
Source
Virology, 2015, 476, p. 1-10 ISSN 0042-6822
Transmission of chikungunya virus (CHIKV) to humans is initiated by puncture of the skin by a blood-feeding Aedes mosquito. Despite the growing knowledge accumulated on CHIKV, the interplay between skin cells and CHIKV following inoculation still remains unclear. In this study we questioned the behavior of human keratinocytes, the predominant cell population in the skin, following viral challenge. We report that CHIKV rapidly elicits an innate immune response in these cells leading to the enhanced transcription of type I/II and type III interferon genes. Concomitantly, we show that despite viral particles internalization into Rab5-positive endosomes and efficient fusion of virus and cell membranes, keratinocytes poorly replicate CHIKV as attested by absence of nonstructural proteins and genomic RNA synthesis. Accordingly, human keratinocytes behave as an antiviral defense against CHIKV infection rather than as a primary targets for initial replication. This picture significantly differs from that reported for Dengue and West Nile mosquito-borne viruses.
Plan de classement
Epidémiologie des arbovirus [052CULARB03]
Descripteurs
ARBOVIROSE ; TRANSMISSION ; INFECTION ; BIOLOGIE MOLECULAIRE ; VECTEUR ; MOUSTIQUE ; IMMUNITE ; ANTIGENE ; CHIKUNGUNYA ; PEAU ; KERATINOCYTE
Localisation
Fonds IRD [F B010063328]
Identifiant IRD
fdi:010063328
Contact