@article{fdi:010063328,
  title = {{H}uman keratinocytes restrict chikungunya virus replication at a post-fusion step},
  author = {{B}ernard, {E}. and {H}amel, {R}odolphe and {N}eyret, {A}. and {E}kchariyawat, {P}. and {M}ol{\`e}s, {J}.{P}. and {S}immons, {G}. and {C}hazal, {N}. and {D}espr{\`e}s, {P}. and {M}iss{\'e}, {D}oroth{\'e}e and {B}riant, {L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}ransmission of chikungunya virus ({CHIKV}) to humans is initiated by puncture of the skin by a blood-feeding {A}edes mosquito. {D}espite the growing knowledge accumulated on {CHIKV}, the interplay between skin cells and {CHIKV} following inoculation still remains unclear. {I}n this study we questioned the behavior of human keratinocytes, the predominant cell population in the skin, following viral challenge. {W}e report that {CHIKV} rapidly elicits an innate immune response in these cells leading to the enhanced transcription of type {I}/{II} and type {III} interferon genes. {C}oncomitantly, we show that despite viral particles internalization into {R}ab5-positive endosomes and efficient fusion of virus and cell membranes, keratinocytes poorly replicate {CHIKV} as attested by absence of nonstructural proteins and genomic {RNA} synthesis. {A}ccordingly, human keratinocytes behave as an antiviral defense against {CHIKV} infection rather than as a primary targets for initial replication. {T}his picture significantly differs from that reported for {D}engue and {W}est {N}ile mosquito-borne viruses.},
  keywords = {{ARBOVIROSE} ; {TRANSMISSION} ; {INFECTION} ; {BIOLOGIE} {MOLECULAIRE} ; {VECTEUR} ; {MOUSTIQUE} ; {IMMUNITE} ; {ANTIGENE} ; {CHIKUNGUNYA} ; {PEAU} ; {KERATINOCYTE}},
  booktitle = {},
  journal = {{V}irology},
  volume = {476},
  numero = {},
  pages = {1--10},
  ISSN = {0042-6822},
  year = {2015},
  DOI = {10.1016/j.virol.2014.11.013},
  URL = {https://www.documentation.ird.fr/hor/fdi:010063328},
}