Publications des scientifiques de l'IRD

Almelli T., Tuikue Ndam Nicaise, Ezimegnon S., Alao M.J., Ahouansou C., Sagbo G., Amoussou A., Deloron Philippe, Tahar Rachida. (2014). Cytoadherence phenotype of Plasmodium falciparum-infected erythrocytes is associated with specific pfemp-1 expression in parasites from children with cerebral malaria. Malaria Journal, 13 (1), art. 333 [9 p. en ligne]. ISSN 1475-2875.

Titre du document
Cytoadherence phenotype of Plasmodium falciparum-infected erythrocytes is associated with specific pfemp-1 expression in parasites from children with cerebral malaria
Année de publication
2014
Type de document
Article
Auteurs
Almelli T., Tuikue Ndam Nicaise, Ezimegnon S., Alao M.J., Ahouansou C., Sagbo G., Amoussou A., Deloron Philippe, Tahar Rachida
Source
Malaria Journal, 2014, 13 (1), art. 333 [9 p. en ligne] ISSN 1475-2875
Background: Cytoadherence of Plasmodium falciparum-infected erythrocytes (IEs) in deep microvasculature endothelia plays a major role in the pathogenesis of cerebral malaria (CM). This biological process is thought to be mediated by P. falciparum erythrocyte membrane protein-1 (PfEMP-1) and human receptors such as CD36 and ICAM-1. The relationship between the expression of PfEMP-1 variants and cytoadherence phenotype in the pathology of malaria is not well established. Methods: Cytoadherence phenotypes of IEs to CD36, ICAM-1, CSPG and the transcription patterns of A, B, var2csa, var3, var gene groups and domain cassettes DC8 and DC13 were assessed in parasites from children with CM and uncomplicated malaria (UM) to determine if cytoadherence is related to a specific transcription profile of pfemp-1 variants. Results: Parasites from CM patients bind significantly more to CD36 than those from UM patients, but no difference was observed in their binding ability to ICAM-1 and CSPG. CM isolates highly transcribed groups A, B, var2csa, var3, DC8 and DC13 compared to UM parasites. The high transcription levels of var genes belonging to group B positively correlated with increased binding level to CD36. Conclusion: CM isolates bind significantly more to CD36 than to ICAM-1, which was correlated with high transcription level of group B var genes, supporting their implication in malaria pathogenesis.
Plan de classement
Lutte [052ANOPAL04]
Descripteurs
PALUDISME ; ENFANT ; PARASITE ; MICROORGANISME ; PHENOTYPE ; CYTOADHERENCE ; ANALYSE GENETIQUE ; TECHNIQUE RT PCR
Description Géographique
BENIN ; COTONOU
Localisation
Fonds IRD [F B010062833]
Identifiant IRD
fdi:010062833
Contact