Publications des scientifiques de l'IRD

Niino T., Wunna, Watanabe K., Nohara N., Rafique T., Yamamoto S., Fukui K., Arizaga M. V., Martinez C. R. C., Matsumoto T., Engelmann Florent. (2014). Cryopreservation of mat rush lateral buds by air dehydration using aluminum cryo-plate. Plant Biotechnology, 31 (3), p. 281-287. ISSN 1342-4580.

Titre du document
Cryopreservation of mat rush lateral buds by air dehydration using aluminum cryo-plate
Année de publication
2014
Type de document
Article référencé dans le Web of Science WOS:000342745200011
Auteurs
Niino T., Wunna, Watanabe K., Nohara N., Rafique T., Yamamoto S., Fukui K., Arizaga M. V., Martinez C. R. C., Matsumoto T., Engelmann Florent
Source
Plant Biotechnology, 2014, 31 (3), p. 281-287 ISSN 1342-4580
A cryopreservation protocol based on air dehydration of explants placed on aluminium cryo-plates, termed D cryo-plate, was successfully developed for in vitro mat rush (Juncus decipiens Nakai) lateral buds. The buds of line 'Hiroshima 4gou(1)' with basal stems were dissected from multiple shoots and precultured overnight at 25 degrees C on solid MS medium containing 0.3 M sucrose. Precultured buds were placed on aluminium cryo-plates, each one with 10 elliptical wells (2.5 mm long, 1.5 mm wide and 0.75 mm deep) and embedded in calcium alginate gel with 0.4 M sucrose and 1 M glycerol. Osmoprotection was performed by immersing the cryo-plates with buds for 30 min at 25 degrees C in loading solution (2 M glycerol + 1.0 M sucrose). Buds were dehydrated to 26% moisture content (fresh weight) by placing the cryo-plates for 2.5 h either in the air current of a laminar flow cabinet or in Petri dishes containing silica gel. Cooling was performed by placing the cryo-plates in uncapped cryotubes, which were immersed in liquid nitrogen. For rewarming, cryo-plates were immersed in liquid medium with 1.0 M sucrose for 15 min at room temperature. Under these conditions, regrowth of cryopreserved buds of line, 'Hiroshima 4gou (1)' was 93% after four weeks culture. The average regrowth of 20 mat rush lines was 88%. The D cryo-plate procedure will facilitate cryostorage of mat rush germplasm.
Plan de classement
Sciences du monde végétal [076] ; Biotechnologies [084]
Localisation
Fonds IRD [F B010062594]
Identifiant IRD
fdi:010062594
Contact