@article{fdi:010062594,
  title = {{C}ryopreservation of mat rush lateral buds by air dehydration using aluminum cryo-plate},
  author = {{N}iino, {T}. and {W}unna, and {W}atanabe, {K}. and {N}ohara, {N}. and {R}afique, {T}. and {Y}amamoto, {S}. and {F}ukui, {K}. and {A}rizaga, {M}. {V}. and {M}artinez, {C}. {R}. {C}. and {M}atsumoto, {T}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{A} cryopreservation protocol based on air dehydration of explants placed on aluminium cryo-plates, termed {D} cryo-plate, was successfully developed for in vitro mat rush ({J}uncus decipiens {N}akai) lateral buds. {T}he buds of line '{H}iroshima 4gou(1)' with basal stems were dissected from multiple shoots and precultured overnight at 25 degrees {C} on solid {MS} medium containing 0.3 {M} sucrose. {P}recultured buds were placed on aluminium cryo-plates, each one with 10 elliptical wells (2.5 mm long, 1.5 mm wide and 0.75 mm deep) and embedded in calcium alginate gel with 0.4 {M} sucrose and 1 {M} glycerol. {O}smoprotection was performed by immersing the cryo-plates with buds for 30 min at 25 degrees {C} in loading solution (2 {M} glycerol + 1.0 {M} sucrose). {B}uds were dehydrated to 26% moisture content (fresh weight) by placing the cryo-plates for 2.5 h either in the air current of a laminar flow cabinet or in {P}etri dishes containing silica gel. {C}ooling was performed by placing the cryo-plates in uncapped cryotubes, which were immersed in liquid nitrogen. {F}or rewarming, cryo-plates were immersed in liquid medium with 1.0 {M} sucrose for 15 min at room temperature. {U}nder these conditions, regrowth of cryopreserved buds of line, '{H}iroshima 4gou (1)' was 93% after four weeks culture. {T}he average regrowth of 20 mat rush lines was 88%. {T}he {D} cryo-plate procedure will facilitate cryostorage of mat rush germplasm.},
  keywords = {{A}ir dehydration ; aluminium plate ; cryo-plate ; {D} cryo-plate method ; mat ; rush},
  booktitle = {},
  journal = {{P}lant {B}iotechnology},
  volume = {31},
  numero = {3},
  pages = {281--287},
  ISSN = {1342-4580},
  year = {2014},
  DOI = {10.5511/plantbiotechnology.14.0624a},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062594},
}