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Belkacem-Hanfi N., Fhoula I., Semmar N., Guesmi A., Perraud Gaime Isabelle, Ouzari H. I., Boudabous A., Roussos Sevastianos. (2014). Lactic acid bacteria against post-harvest moulds and ochratoxin A isolated from stored wheat. Biological Control, 76, p. 52-59. ISSN 1049-9644.

Titre du document
Lactic acid bacteria against post-harvest moulds and ochratoxin A isolated from stored wheat
Année de publication
2014
Type de document
Article référencé dans le Web of Science WOS:000339538800007
Auteurs
Belkacem-Hanfi N., Fhoula I., Semmar N., Guesmi A., Perraud Gaime Isabelle, Ouzari H. I., Boudabous A., Roussos Sevastianos
Source
Biological Control, 2014, 76, p. 52-59 ISSN 1049-9644
A total of 54 lactic acid bacteria (LAB) were isolated from stored wheat samples sourced from grain silos in North Tunisia. Fifteen representative isolates were identified by 16S rDNA sequencing as Pediococcus pentosaceus, Lactobacillus plantarum, Lactobacillus graminis, Lactobacillus coryniformis and Weissella cibaria. These isolates were screened for antifungal activity in dual culture agar plate assay against eight post-harvest moulds (Penicillium expansum, Penicillium chrysogenum, Penicillium glabrum, Aspergillus flavus, Aspergillus niger, Aspergillus carbonarius, Fusarium graminearum and Alternaria alternata). All LAB showed inhibitory activity against moulds, especially strains of L. plantarum which exhibited a large antifungal spectrum. Moreover, LAB species such as L plantarum LabN10, L. graminis LabN11 and P. pentosaceus LabN12 showed high inhibitory effects against the ochratoxigenic strain A. carbonarius ANC89. These LAB were also investigated for their ability to reduce A. carbonarius ANC89 biomass and its ochratoxin A (OTA) production on liquid medium at 28 and 37 degrees C and varied pH conditions. The results indicated that factors such as temperature, pH and bacterial biomass on mixed cultures, has a significant effect on fungal inhibition and OTA production. High percentage of OTA reduction was obtained by L. plantarum and L. graminis (>97%) followed by P. pentosaceus (>81.5%). These findings suggest that in addition to L. plantarum, L graminis and P. pentosaceus strains may be exploited as a potential OTA detoxifying agent to protect humans and animals health against this toxic metabolite.
Plan de classement
Nutrition, alimentation [054] ; Biotechnologies [084]
Description Géographique
TUNISIE
Localisation
Fonds IRD [F B010062400]
Identifiant IRD
fdi:010062400
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