Publications des scientifiques de l'IRD

Ferretto N., Tedetti Marc, Guigue C., Mounier S., Redon R., Goutx M. (2014). Identification and quantification of known polycyclic aromatic hydrocarbons and pesticides in complex mixtures using fluorescence excitation–emission matrices and parallel factor analysis. Chemosphere, 107, p. 344-353. ISSN 0045-6535.

Titre du document
Identification and quantification of known polycyclic aromatic hydrocarbons and pesticides in complex mixtures using fluorescence excitation–emission matrices and parallel factor analysis
Année de publication
2014
Type de document
Article référencé dans le Web of Science WOS:000337929600044
Auteurs
Ferretto N., Tedetti Marc, Guigue C., Mounier S., Redon R., Goutx M.
Source
Chemosphere, 2014, 107, p. 344-353 ISSN 0045-6535
Polycyclic aromatic hydrocarbons (PAHs) and pesticides are among the most widespread organic contaminants in aquatic environments. Because of their aromatic structure, PAHs and pesticides have intrinsic fluorescence properties in the ultraviolet/blue spectral range. In this study, excitation–emission matrix (EEM) fluorescence spectroscopy and parallel factor (PARAFAC) analysis were used to characterise and discriminate fluorescence signatures of nine PAHs and three pesticides at the µg L-1 level in the presence of humic substances (0.1-10 mg C L-1). These contaminants displayed a diversity of fluorescence signatures regarding spectral position (λEx: 220-335 nm, λEm: 310-414 nm), Stokes shift (39-169 nm) and number of peaks (1-8), with detection limits ranging from 0.02 to 1.29 µg L-1. The EEM/PARAFAC method applied to mixtures of PAHs with humic substances validated a seven-component model that included one humic-like fluorophore and six PAH-like fluorophores. The EEM/PARAFAC method applied to mixtures of pesticides with humic substances validated a six-component model that included one humic-like fluorophore and three pesticide-like fluorophores. The EEM/PARAFAC method adequately quantified most of the contaminants for humic substance concentrations not exceeding 2.5 mg C L-1. The application of this method to natural (marine) samples was demonstrated through (1) the match between the Ex and Em spectra of PARAFAC components and the Ex and Em spectra of standard PAHs, and (2) the good linear correlations between the fluorescence intensities of PARAFAC components and the PAH concentrations determined by GC-MS.
Plan de classement
Physique / Chimie [020PHYCHI] ; Pollution océanique [038POLOCE]
Descripteurs
POLLUTION MARINE ; MILIEU AQUATIQUE ; MER ; PESTICIDE ; CONTAMINATION ; FLUORESCENCE ; SPECTROSCOPIE ; HUMUS ; ETUDE COMPARATIVE ; HAP.HYDROCARBURE AROMATIQUE POLYCYCLIQUE
Identifiant IRD
fdi:010061696
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