Horizon / Plein textes La base de ressources documentaires de l'IRD



Publications des scientifiques de l'IRD

Ngoyi D. M., Menten J., Pyana P. P., Buscher P., Lejon Veerle. (2013). Stage determination in sleeping sickness : comparison of two cell counting and two parasite detection techniques. Tropical Medicine and International Health, 18 (6), 778-782. ISSN 1360-2276

Accès réservé (Intranet IRD) Demander le PDF

Lien direct chez l'éditeur doi:10.1111/tmi.12102

Stage determination in sleeping sickness : comparison of two cell counting and two parasite detection techniques
Année de publication2013
Type de documentArticle référencé dans le Web of Science WOS:000320334600014
AuteursNgoyi D. M., Menten J., Pyana P. P., Buscher P., Lejon Veerle.
SourceTropical Medicine and International Health, 2013, 18 (6), p. 778-782. ISSN 1360-2276
RésuméOBJECTIVES Diagnosis of the neurological stage of human African trypanosomiasis is performed by examination of cerebrospinal fluid (CSF) for the presence of trypanosomes and numbers of white blood cells (WBC). Both CSF parameters are also used to assess treatment outcome during follow-up. In view of the importance of CSF examination, and the practical problems encountered with it, we compared the sensitivity of two trypanosome concentration techniques and the repeatability of two cell counting METHODS, as well as occurrence of systematic differences between them. methods Patients were recruited at Dipumba hospital, in Mbuji-Mayi in the Democratic Republic of the Congo. In 94 CSF samples, trypanosome detection was performed with modified single centrifugation (MSC) and double centrifugation (DC). On 189 CSF samples with <= 30 cells/mu l, cell counting was performed in duplicate in a Fuchs-Rosenthal counting chamber and in a disposable Uriglass counting chamber. RESULTS Modified single centrifugation detected trypanosomes in significantly (P < 0.0001) more patients (85) than DC (46). Cell counts did not differ systematically in the two methods. Variability in the differences between duplicate cell counts was significantly higher (P = 0.002) in Uriglass (SD of differences 2.03) than in Fuchs-Rosenthal (SD of differences 1.62). CONCLUSIONS For analysis of CSF in the context of sleeping sickness stage determination and follow-up after treatment, we strongly recommend the MSC for parasite detection and the application of disposable counting chambers. When the first cell count is <= 20 cells/mu l, we recommend repeating the counting procedure on the same CSF specimen and taking the average of both countings.
Plan de classementEntomologie médicale / Parasitologie / Virologie [052]
Descr. géo.AFRIQUE
LocalisationFonds IRD [F B010060430]
Identifiant IRDfdi:010060430
Lien permanenthttp://www.documentation.ird.fr/hor/fdi:010060430

Export des données

Disponibilité des documents

Télechargment fichier PDF téléchargeable

Lien sur le Web lien chez l'éditeur

Accès réservé en accès réservé

HAL en libre accès sur HAL

Accès aux documents originaux :

Le FDI est labellisé CollEx

Accès direct

Bureau du chercheur

Site de la documentation

Espace intranet IST (accès réservé)

Suivi des publications IRD (accès réservé)

Mentions légales

Services Horizon

Poser une question

Consulter l'aide en ligne

Déposer une publication (accès réservé)

S'abonner au flux RSS

Voir les tableaux chronologiques et thématiques

Centres de documentation


Montpellier (centre IRD)

Montpellier (MSE)









La Paz