@article{fdi:010060430,
  title = {{S}tage determination in sleeping sickness : comparison of two cell counting and two parasite detection techniques},
  author = {{N}goyi, {D}. {M}. and {M}enten, {J}. and {P}yana, {P}. {P}. and {B}uscher, {P}. and {L}ejon, {V}eerle},
  editor = {},
  language = {{ENG}},
  abstract = {{OBJECTIVES} {D}iagnosis of the neurological stage of human {A}frican trypanosomiasis is performed by examination of cerebrospinal fluid ({CSF}) for the presence of trypanosomes and numbers of white blood cells ({WBC}). {B}oth {CSF} parameters are also used to assess treatment outcome during follow-up. {I}n view of the importance of {CSF} examination, and the practical problems encountered with it, we compared the sensitivity of two trypanosome concentration techniques and the repeatability of two cell counting {METHODS}, as well as occurrence of systematic differences between them. methods {P}atients were recruited at {D}ipumba hospital, in {M}buji-{M}ayi in the {D}emocratic {R}epublic of the {C}ongo. {I}n 94 {CSF} samples, trypanosome detection was performed with modified single centrifugation ({MSC}) and double centrifugation ({DC}). {O}n 189 {CSF} samples with <= 30 cells/mu l, cell counting was performed in duplicate in a {F}uchs-{R}osenthal counting chamber and in a disposable {U}riglass counting chamber. {RESULTS} {M}odified single centrifugation detected trypanosomes in significantly ({P} < 0.0001) more patients (85) than {DC} (46). {C}ell counts did not differ systematically in the two methods. {V}ariability in the differences between duplicate cell counts was significantly higher ({P} = 0.002) in {U}riglass ({SD} of differences 2.03) than in {F}uchs-{R}osenthal ({SD} of differences 1.62). {CONCLUSIONS} {F}or analysis of {CSF} in the context of sleeping sickness stage determination and follow-up after treatment, we strongly recommend the {MSC} for parasite detection and the application of disposable counting chambers. {W}hen the first cell count is <= 20 cells/mu l, we recommend repeating the counting procedure on the same {CSF} specimen and taking the average of both countings.},
  keywords = {sleeping sickness ; cerebrospinal fluid ; {T}rypanosoma brucei gambiense ; human {A}frican trypanosomiasis ; {AFRIQUE}},
  booktitle = {},
  journal = {{T}ropical {M}edicine and {I}nternational {H}ealth},
  volume = {18},
  numero = {6},
  pages = {778--782},
  ISSN = {1360-2276},
  year = {2013},
  DOI = {10.1111/tmi.12102},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060430},
}