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Dubourg G., Lagier J. C., Armougom Fabrice, Robert C., Audoly G., Papazian L., Raoult Didier. (2013). High-level colonisation of the human gut by Verrucomicrobia following broad-spectrum antibiotic treatment. International Journal of Antimicrobial Agents, 41 (2), 149-155. ISSN 0924-8579

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Lien direct chez l'éditeur doi:10.1016/j.ijantimicag.2012.10.012

Titre
High-level colonisation of the human gut by Verrucomicrobia following broad-spectrum antibiotic treatment
Année de publication2013
Type de documentArticle référencé dans le Web of Science WOS:000313669600009
AuteursDubourg G., Lagier J. C., Armougom Fabrice, Robert C., Audoly G., Papazian L., Raoult Didier.
SourceInternational Journal of Antimicrobial Agents, 2013, 41 (2), p. 149-155. ISSN 0924-8579
RésuméThe gut microbiota is mainly composed of the phyla Firmicutes, Bacteroidetes, Actinobacteria and Proteobacteria; the Verrucomicrobia phylum is occasionally observed. Antibiotics can change the bacterial diversity of the gut, with limited changes in the proportions of phyla. In this study, the gut repertoire of two patients who received a broad-spectrum antibiotic regimen was studied. As part of a large gut microbiota study, two stool samples were analysed: one sample was collected after broad-spectrum antibiotic therapy in a patient with Coxiella burnetii vascular infection (Patient A); and the other sample was collected from a patient admitted to the Intensive Care Unit (Patient B). Samples were subjected to Gram staining, electron microscopy, 16S rRNA V6 amplicon pyrosequencing and fluorescence in situ hybridisation (FISH). In parallel, the antibiotic susceptibility of Akkermansia muciniphila Muc(T) strain was studied and this strain was observed by electron microscopy. Pyrosequencing revealed that a large proportion of the sequences were associated with Verrucomicrobia (proportions of 44.9% and 84.6% for Patients A and B, respectively). All of the phylotypes were represented by a single species (A. muciniphila), and neither patient presented significant gastrointestinal disorders. Electron microscopy and FISH with specific Verrucomicrobia probes confirmed the presence of the bacterium. The Muc(T) strain was susceptible to imipenem and doxycycline but resistant to vancomycin and metronidazole. Dramatic colonisation of the human gut microbiota by the Verrucomicrobia phylum following a broad-spectrum antibiotic regimen occurred without significant gastrointestinal manifestations, suggesting that influenced by external factors such as antibiotics, the gut repertoire remains partially unknown.
Plan de classementBiotechnologies [084] ; Santé : généralités [050] ; Entomologie médicale / Parasitologie / Virologie [052]
Descr. géo.FRANCE
LocalisationFonds IRD [F B010058870]
Identifiant IRDfdi:010058870
Lien permanenthttp://www.documentation.ird.fr/hor/fdi:010058870

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