@article{fdi:010058870,
  title = {{H}igh-level colonisation of the human gut by {V}errucomicrobia following broad-spectrum antibiotic treatment},
  author = {{D}ubourg, {G}. and {L}agier, {J}. {C}. and {A}rmougom, {F}abrice and {R}obert, {C}. and {A}udoly, {G}. and {P}apazian, {L}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he gut microbiota is mainly composed of the phyla {F}irmicutes, {B}acteroidetes, {A}ctinobacteria and {P}roteobacteria; the {V}errucomicrobia phylum is occasionally observed. {A}ntibiotics can change the bacterial diversity of the gut, with limited changes in the proportions of phyla. {I}n this study, the gut repertoire of two patients who received a broad-spectrum antibiotic regimen was studied. {A}s part of a large gut microbiota study, two stool samples were analysed: one sample was collected after broad-spectrum antibiotic therapy in a patient with {C}oxiella burnetii vascular infection ({P}atient {A}); and the other sample was collected from a patient admitted to the {I}ntensive {C}are {U}nit ({P}atient {B}). {S}amples were subjected to {G}ram staining, electron microscopy, 16{S} r{RNA} {V}6 amplicon pyrosequencing and fluorescence in situ hybridisation ({FISH}). {I}n parallel, the antibiotic susceptibility of {A}kkermansia muciniphila {M}uc({T}) strain was studied and this strain was observed by electron microscopy. {P}yrosequencing revealed that a large proportion of the sequences were associated with {V}errucomicrobia (proportions of 44.9% and 84.6% for {P}atients {A} and {B}, respectively). {A}ll of the phylotypes were represented by a single species ({A}. muciniphila), and neither patient presented significant gastrointestinal disorders. {E}lectron microscopy and {FISH} with specific {V}errucomicrobia probes confirmed the presence of the bacterium. {T}he {M}uc({T}) strain was susceptible to imipenem and doxycycline but resistant to vancomycin and metronidazole. {D}ramatic colonisation of the human gut microbiota by the {V}errucomicrobia phylum following a broad-spectrum antibiotic regimen occurred without significant gastrointestinal manifestations, suggesting that influenced by external factors such as antibiotics, the gut repertoire remains partially unknown.},
  keywords = {{G}ut microbiota ; {A}kkermansia muciniphila ; {A}ntibiotics ; {P}yrosequencing ; {FRANCE}},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {A}ntimicrobial {A}gents},
  volume = {41},
  numero = {2},
  pages = {149--155},
  ISSN = {0924-8579},
  year = {2013},
  DOI = {10.1016/j.ijantimicag.2012.10.012},
  URL = {https://www.documentation.ird.fr/hor/fdi:010058870},
}