Dobigny Gauthier, Poirier P., Hima Karmadine, Cabaret O., Gauthier Philippe, Tatard C., Costa J. M., Bretagne S. (2011). Molecular survey of rodent-borne Trypanosoma in Niger with special emphasis on T. lewisi imported by invasive black rats. Acta Tropica, 117 (3), p. 183-188. ISSN 0001-706X.
Titre du document
Molecular survey of rodent-borne Trypanosoma in Niger with special emphasis on T. lewisi imported by invasive black rats
Dobigny Gauthier, Poirier P., Hima Karmadine, Cabaret O., Gauthier Philippe, Tatard C., Costa J. M., Bretagne S.
Source
Acta Tropica, 2011,
117 (3), p. 183-188 ISSN 0001-706X
Invading rodent species can harbor parasites with potential transmission to native rodents and/or humans. To investigate trypanosomes prevalence in rodents, the spleen of 76 rodents from Niger identified by their karyotype was used as a DNA source for Trypanosoma detection using a newly developed qPCR assay. Of the invasive black rat, Rattus rattus, 71% (10/14) were PCR positive as well as 6% (4/62) of native African rodents. Sequences of similar to 400 bp of the SSU rDNA gene identified phylogenetically close Trypanosoma lineages. Trypanosoma lewisi was present in all positive black rats and the sequences displayed 100% similarity with T. lewisi-infected humans in Senegal. T. lewisi was also detected in one Acomys johannis, suggesting a possible transmission to native species. In addition to improved knowledge of Trypanosoma diversity in rodents, our data underscore the introduction of the potentially pathogenic T. lewisi kinetoplastid through the human-mediated invasion of black rats all over West Africa.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052]
;
Sciences du monde animal [080]
