Doignon-Bourcier F., Laguerre G., Willems A., Sy L., Dreyfus Bernard, Lajudie Philippe de, Gillis M. (1999). Genotypic characterization of bradyrhizobia from small legumes by rDNA PCR-RFLP and AFLP fingerprint analyses. In :
Martinez E. (ed.), Hernandez G. (ed.). Highlights of nitrogen fixation research. New York : Kluwer, p. 281-289 multigr. Symbiotic Nitrogen Fixation : North American Conference, 16., Cancun (MEX), 1998/02/01-06. ISBN 0-306-46137-4.
Titre du document
Genotypic characterization of bradyrhizobia from small legumes by rDNA PCR-RFLP and AFLP fingerprint analyses
Année de publication
1999
Type de document
Littérature grise
Auteurs
Doignon-Bourcier F., Laguerre G., Willems A., Sy L., Dreyfus Bernard, Lajudie Philippe de, Gillis M.
In
Martinez E. (ed.), Hernandez G. (ed.), Highlights of nitrogen fixation research
Source
New York : Kluwer, 1999,
p. 281-289 multigr. ISBN 0-306-46137-4
Colloque
Symbiotic Nitrogen Fixation : North American Conference, 16., Cancun (MEX), 1998/02/01-06
At the present time, the precise taxonomial status of many strains classified as Bradyrhizobium still remains unclear. There is a need to develop a reliable grouping method, specifying the genetic relationships between these strains. Phenotypic methods as auxanography or protein profiling did not prove valuable for classification of bradyrhizobia and were not in good agreement with phylogenetic data. The purpose of this work is to develop a strategy to analyse the diversity of bradyrhizobia and to identify genomic groups among our collection of strains isolated from 9 small legume species in Senegal. B. japonicum, B. elkanii and representatives of previously described groups by Moreira et al. (1993) and Dupuy et al. (1994) were included as references. Bacterial diversity was assessed by two different techniques, PCR-RFLP analysis of the IGS region between 16S and 23S rDNA (IGS) and the AFLP technique. Groupings of strains obtained by the two methodologies will be presented and compared. 16S rDNA PCR-RFLP analysis of strains representative of IGS clusters from small legumes was also performed. (Résumé d'auteur)
Plan de classement
Fixation biologique de l'azote [084SYMBIO02]
Descripteurs
LEGUMINEUSE TROPICALE ; BACTERIE ; NODULE RACINAIRE ; FIXATION BIOLOGIQUE DE L'AZOTE ; ANALYSE GENETIQUE ; GENOTYPE ; TECHNIQUE PCR ; TECHNIQUE RFLP ; ETUDE EXPERIMENTALE ; ETUDE COMPARATIVE ; TECHNIQUE AFLP
Description Géographique
SENEGAL
Localisation
Fonds IRD [F B010023999] ;
Montpellier (Centre IRD)
Identifiant IRD
fdi:010023999
