Ka M. B., Mezouar S., Ben Amara A., Raoult Didier, Ghigo E., Olive D., Mege J. L. (2016). Coxiella burnetii induces inflammatory interferon-like signature in plasmacytoid dendritic cells : a new feature of immune response in Q fever. Frontiers in Cellular and Infection Microbiology, 6, p. 70. ISSN 2235-2988.
Titre du document
Coxiella burnetii induces inflammatory interferon-like signature in plasmacytoid dendritic cells : a new feature of immune response in Q fever
Ka M. B., Mezouar S., Ben Amara A., Raoult Didier, Ghigo E., Olive D., Mege J. L.
Source
Frontiers in Cellular and Infection Microbiology, 2016,
6, p. 70 ISSN 2235-2988
Plasmacytoid dendritic cells (pDCs) play a major role in antiviral immunity via the production of type I interferons (IFNs). There is some evidence that pDCs interact with bacteria but it is not yet clear whether they are protective or contribute to bacterial pathogenicity. We wished to investigate whether Coxiella burnetii, the agent of Q fever, interacts with pDCs. The stimulation of pDCs with C. burnetii increased the expression of activation and migratory markers (CD86 and CCR7) as determined by flow cytometry and modulated gene expression program as revealed by a microarray approach. Indeed, genes encoding for pro-inflammatory cytokines, chemokines, and type I INF were up-regulated. The up-regulation of type I IFN was correlated with an increase in IFN-alpha release by C. burnetii-stimulated pDCs. We also investigated pDCs in patients with Q fever endocarditis. Using flow cytometry and a specific gating strategy, we found that the number of circulating pDCs was significantly lower in patients with Q fever endocarditis as compared to healthy donors. In addition, the remaining circulating pDCs expressed activation and migratory markers. As a whole, our study identified non-previously reported activation of pDCs by C. bumetil and their modulation during Q fever.
