Non contiguous-finished genome sequence and description of Bacillus jeddahensis sp. nov.

2015

Article référencé dans le Web of Science WOS:000367991300001

Bittar F., Bibi F., Ramasamy D., Lagier J. C., Azhar E. I., Jiman-Fatani A. A., Al-Ghamdi A. K., Nguyen T. T., Yasir M., Fournier P. E., Raoult Didier

Standards in Genomic Sciences, 2015, 10, p. art. 47 ISSN 1944-3277

Strain JCE(T) was isolated from the fecal sample of a 24-year-old obese man living in Jeddah, Saudi Arabia. It is an aerobic, Gram-positive, rod-shaped bacterium. This strain exhibits a 16S rRNA nucleotide sequence similarity of 97.5 % with Bacillus niacini, the phylogenetically closest species with standing nomenclature. Moreover, the strain JCE(T) presents many phenotypic differences, when it is compared to other Bacillus species, and shows a low MALDI-TOF Mass Spectrometry score that does not allow any identification. Thus, it is likely that this strain represents a new species. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 4,762,944 bp long genome (1 chromosome but no plasmid) contains 4,654 protein-coding and 98 RNAs genes, including 92 tRNA genes. The strain JCE(T) differs from most of the other closely Bacillus species by more than 1 % in G + C content. In addition, digital DNA-DNA hybridization values for the genome of the strain JCE(T) against the closest Bacillus genomes range between 19.5 to 28.1, that confirming again its new species status. On the basis of these polyphasic data made of phenotypic and genomic analyses, we propose the creation of Bacillus jeddahensis sp. nov. that contains the strain JCE(T).

Sciences fondamentales / Techniques d'analyse et de recherche [020] ; Biotechnologies [084]

ARABIE SAOUDITE

Fonds IRD

PAR00014192