Edouard S., Prudent E., Gautret P., Memish Z. A., Raoult Didier. (2015). Cost-Effective Pooling of DNA from Nasopharyngeal Swab Samples for Large-Scale Detection of Bacteria by Real-Time PCR. Journal of Clinical Microbiology, 53 (3), p. 1002-1004. ISSN 0095-1137.
Titre du document
Cost-Effective Pooling of DNA from Nasopharyngeal Swab Samples for Large-Scale Detection of Bacteria by Real-Time PCR
Edouard S., Prudent E., Gautret P., Memish Z. A., Raoult Didier
Source
Journal of Clinical Microbiology, 2015,
53 (3), p. 1002-1004 ISSN 0095-1137
We investigated the potential of pooling DNA from nasopharyngeal specimens to reduce the cost of real-time PCR (RT-PCR) for bacterial detection. Lyophilization is required to reconcentrate DNA. This strategy yields a high specificity (86%) and a high sensitivity (96%). We estimate that compared to individual testing, 37% fewer RT-PCR tests are needed.
