%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Edouard, S.
%A Prudent, E.
%A Gautret, P.
%A Memish, Z. A.
%A Raoult, Didier
%T Cost-Effective Pooling of DNA from Nasopharyngeal Swab Samples for Large-Scale Detection of Bacteria by Real-Time PCR
%D 2015
%L PAR00012934
%G ENG
%J Journal of Clinical Microbiology
%@ 0095-1137
%M ISI:000350204600040
%N 3
%P 1002-1004
%R 10.1128/jcm.03609-14
%U https://www.documentation.ird.fr/hor/PAR00012934
%V 53
%W Horizon (IRD)
%X We investigated the potential of pooling DNA from nasopharyngeal specimens to reduce the cost of real-time PCR (RT-PCR) for bacterial detection. Lyophilization is required to reconcentrate DNA. This strategy yields a high specificity (86%) and a high sensitivity (96%). We estimate that compared to individual testing, 37% fewer RT-PCR tests are needed.
%$ 084