%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Cerapio, J.P.
%A Contreras-Mancilla, J.
%A Fernandez, R.
%A Fournie, J.J.
%A Deharo, Eric
%A Bertani, Stéphane
%A Pineau, P.
%A Casavilca-Zambrano, S.
%A Ruiz, E.
%T Cancer de higado : antigua o nueva amenaza para los jovenes de paises en via de desarrollo ?
%D 2022
%L fdi:010084427
%G SPA
%J Revista Peruana de Ciencias de la Salud
%@ 2707-6946
%K PEROU
%N 1
%P 5-8
%R 10.37711/rpcs.2022.4.1.362
%U https://www.documentation.ird.fr/hor/fdi:010084427
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2023-02/010084427.pdf
%V 4
%W Horizon (IRD)
%X Entre las neoplasias malignas más frecuentes del mundo, el carcinoma hepatocelular (CHC) es la segunda causa de muerte relacionada con el cáncer (1). Su incidencia se ha duplicado durante las dos últimas décadas y la mayor carga se produce en los países de ingresos bajos y medianos. Los tumores hepáticos primarios malignos suelen describirse como una patología que afecta principalmente a hombres mayores de 40 años con un hígado cirrótico; rara vez se han registrado en personas más jóvenes y normalmente, en menores de 40, lo más común es el hepatoblastoma.
%$ 050MEDECI

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Sitbounlang, P.
%A Deharo, Eric
%A Latthaphasavang, Vatthanaphone
%A Marchio, A.
%A Soukhsakhone, C.
%A Soinxay, V.
%A Mayxay, M.
%A Steenkeste, N.
%A Vincelot, P.
%A Bertani, Stéphane
%A Palamy, S.
%A Paboriboune, P.
%A Pineau, P.
%T Estimating the burden of hepatitis B virus infection in Laos between 2020 and 2021 : a cross-sectional seroprevalence survey
%D 2022
%L fdi:010086874
%G ENG
%J eClinicalMedicine
%K Hepatitis B virus ; Southeast Asia ; Laos ; Endemic country ; Universal ; Immunization
%K LAOS
%M ISI:000916607100004
%P 101582 [12 ]
%R 10.1016/j.eclinm.2022.101582
%U https://www.documentation.ird.fr/hor/fdi:010086874
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2023-03/010086874.pdf
%V 52
%W Horizon (IRD)
%X Background Laos is considered highly endemic for persistent infection with hepatitis B virus (HBV). To eliminate this burden, it has gradually implemented universal anti-hepatitis B immunisation of newborns over the past two decades. Methods Using VIKIA (R) HBsAg, a rapid test for the qualitative detection of the HBV surface antigen, we conducted between Sep 1st, 2020 and Aug 31st, 2021 the largest prospective prevalence survey ever in Laos. This survey included blood donors (BD, n = 42,277), patients attending care in capital and provincial hospitals (n = 37,347) including attending mothers (n = 20,548), HIV-infected patients (n = 7439, recruited from 2009 to 2020), students from the Health Sciences University (n = 609), and outpatients (n = 350) coming for diagnosis at the Center Infectiology Lao-Christophe Merieux in Vientiane. In total, 88,022 persons were tested, representing approximately 1.22% of the national population. To reach a reasonable estimate of HBsAg prevalence in Laos, we segmented the population according to three variables, age (=20 years as a cut-off), sex, and geographical origin. BD values were used to estimate HBsAg prevalence in patients aged <20 while hospital survey prevalence was used to estimate the prevalence in those aged older than 20 years. Findings We observed an HBsAg seroprevalence ranging from 2.6% in blood donors to 8.0% in HIV-infected patients. In BD, men were significantly more at risk to be carriers than women (RR = 1.2, P = 0.00063). For BD, attendingmothers, or HIV-infected patients, HBsAg was significantly more prevalent in northern Laos (5.1-8.4%) than in central (2.0-8.1%) or southern parts of the country (2.2- 6.9%), thereby delineating a North-to-South gradient. Interpretation We considered that HBsAg prevalence probably ranges between 5.0% and 6.0% of the total population. Thus, we consider that Laos may no longer be highly endemic for chronic HBV infection but rather a country with intermediate endemicity.
%$ 050 ; 052

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Vidaurre, T.
%A Enriquez-Vera, D.
%A Bertani, Stéphane
%T Excess mortality in patients with cancer during the COVID-19 pandemic in Peru : an analysis of death registry data [résumé]
%D 2022
%L fdi:010085857
%G ENG
%J Lancet Oncology
%@ 1470-2045
%K PEROU
%M ISI:000828572700029
%N Special Issue 1
%P S28
%U https://www.documentation.ird.fr/hor/fdi:010085857
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2022-09/010085857.pdf
%V 23
%W Horizon (IRD)
%B The Lancet Summit. Cancer Care in Asia and Latin America
%8 2022/07/14-15
%$ 050 ; 052

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Honles, J.
%A Clisson, C.
%A Monge, C.
%A Vasquez-Ocmin, P.
%A Cerapio, J. P.
%A Palamy, S.
%A Casavilca-Zambrano, S.
%A Herrera, Javier
%A Pineau, P.
%A Deharo, Eric
%A Peynet, V.
%A Bertani, Stéphane
%T Exposure assessment of 170 pesticide ingredients and derivative metabolites in people from the Central Andes of Peru
%D 2022
%L fdi:010085993
%G ENG
%J Scientific Reports - Nature
%@ 2045-2322
%K PEROU ; ANDES
%M ISI:000837764800008
%N 1
%P 13525 [15 ]
%R 10.1038/s41598-022-17772-1
%U https://www.documentation.ird.fr/hor/fdi:010085993
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2022-09/010085993.pdf
%V 12
%W Horizon (IRD)
%X The Central Andes of Peru are a region of great concern regarding pesticide risk to the health of local communities. Therefore, we conducted an observational study to assess the level of pesticide contamination among Andean people. Analytical chemistry methods were used to measure the concentrations of 170 pesticide-related compounds in hair samples from 50 adult Andean subjects living in rural and urban areas. As part of the study, a questionnaire was administered to the subjects to collect information regarding factors that increase the risk of pesticide exposure. Our results indicate that Andean people are strongly exposed to agrochemicals, being contaminated with a wide array of pesticide-related compounds at high concentration levels. Multivariate analyses and geostatistical modeling identified sociodemographic factors associated with rurality and food origin that increase pesticide exposure risk. The present study represents the first comprehensive investigation of pesticide-related compounds detected in body samples collected from people living in the Central Andes of Peru. Our findings pinpoint an alarming environmental situation that threatens human health in the region and provide a rationale for improving public policies to protect local communities.
%$ 050 ; 038 ; 020

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Fernandez-Placencia, R. M.
%A Montenegro, P.
%A Guerrero, M.
%A Serrano, M.
%A Ortega, E.
%A Bravo, M.
%A Huanca, L.
%A Bertani, Stéphane
%A Trejo, J. M.
%A Webb, P.
%A Malca-Vasquez, J.
%A Taxa, L.
%A Lachos-Davila, A.
%A Celis-Zapata, J.
%A Luque-Vasquez, C.
%A Payet, E.
%A Ruiz, E.
%A Berrospi, F.
%T Survival after curative pancreaticoduodenectomy for ampullary adenocarcinoma in a South American population : a retrospective cohort study
%D 2022
%L fdi:010085215
%G ENG
%J World Journal of Gastrointestinal Surgery
%@ 1948-9366
%K Gastrointestinal neoplasms ; Adenocarcinoma ; Ampulla ; Pancreaticoduodenectomy ; Survival ; South America
%K PEROU
%M ISI:000802504900003
%N 1
%P 24-35
%R 10.4240/wjgs.v14.i1.24
%U https://www.documentation.ird.fr/hor/fdi:010085215
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2022-07/010085215.pdf
%V 14
%W Horizon (IRD)
%X BACKGROUND Ampullary adenocarcinoma (AAC) is a rare neoplasm that accounts for only 0.2% of all gastrointestinal cancers. Its incidence rate is lower than 6 cases per million people. Different prognostic factors have been described for AAC and are associated with a wide range of survival rates. However, these studies have been exclusively conducted in patients originating from Asian, European, and North American countries. AIM To evaluate the histopathologic predictors of overall survival (OS) in South American patients with AAC treated with curative pancreaticoduodenectomy (PD). METHODS We analyzed retrospective data from 83 AAC patients who underwent curative (R0) PD at the National Cancer Institute of Peru between January 2010 and October 2020 to identify histopathologic predictors of OS. RESULTS Sixty-nine percent of patients had developed intestinal-type AAC (69%), 23% had pancreatobiliary-type AAC, and 8% had other subtypes. Forty-one percent of patients were classified as Stage I, according to the AJCC 8th Edition. Recurrence occurred primarily in the liver (n = 8), peritoneum (n = 4), and lung (n = 4). Statistical analyses indicated that T3 tumour stage [hazard ratio (HR) of 6.4, 95% confidence interval (CI) of 2.5-16.3, P < 0.001], lymph node metastasis (HR: 4.5, 95%CI: 1.8-11.3, P = 0.001), and pancreatobiliary type (HR: 2.7, 95%CI: 1.2-6.2, P = 0.025) were independent predictors of OS. CONCLUSION Extended tumour stage (T3), pancreatobiliary type, and positive lymph node metastasis represent independent predictors of a lower OS rate in South American AAC patients who underwent curative PD.
%$ 050

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Ruiz, E.
%A Pineau, P.
%A Flores, C.
%A Fernández, R.
%A Cano, L.
%A Cerapio, J.P.
%A Casavilca-Zambrano, S.
%A Berrospi, F.
%A Chávez, I.
%A Roche, Benjamin
%A Bertani, Stéphane
%T A preoperative nomogram for predicting long-term survival after resection of large hepatocellular carcinoma (sup. 10 cm)
%D 2022
%L fdi:010082443
%G ENG
%J HPB
%@ 1365-182X
%K PEROU
%M ISI:000760320000007
%N 2
%P 192-201
%R 10.1016/j.hpb.2021.06.006
%U https://www.documentation.ird.fr/hor/fdi:010082443
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2021-08/010082443.pdf
%V 24
%W Horizon (IRD)
%X Background: It has previously been demonstrated that a fraction of patients with hepatocellular carcinoma (HCC) sup. 10 cm can benefit from liver resection. However, there is still a lack of effective decision-making tools to inform intervention in these patients. Methods: We analysed a comprehensive set of clinical data from 234 patients who underwent liver resection for HCC sup.10 cm at the National Cancer Institute of Peru between 1990 and 2015, monitored their survival, and constructed a nomogram to predict the surgical outcome based on preoperative variables. Results: We identified cirrhosis, multifocality, macroscopic vascular invasion, and spontaneous tumour rupture as independent predictors of survival and integrated them into a nomogram model. The nomogram's ability to forecast survival at 1, 3, and 5 years was subsequently confirmed with high concordance using an internal validation. Through applying this nomogram, we stratified three groups of patients with different survival probabilities. Conclusion: We constructed a preoperative nomogram to predict long-term survival in patients with HCC sup.10 cm. This nomogram is useful in determining whether a patient with large HCC might truly benefit from liver resection, which is paramount in low- and middle-income countries where HCC is often diagnosed at advanced stages.
%$ 050 ; 020

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Cerapio, J.P.
%A Marchio, A.
%A Cano, L.
%A López, I.
%A Fournié, J.J.
%A Régnault, B.
%A Casavilca-Zambrano, S.
%A Ruiz, E.
%A Dejean, A.
%A Bertani, Stéphane
%A Pineau, P.
%T Global DNA hypermethylation pattern and unique gene expression signature in liver cancer from patients with Indigenous American ancestry
%D 2021
%L fdi:010082444
%G ENG
%J Oncotarget
%@ 1949-2553
%K PEROU
%N 5
%P 475-492
%R 10.18632/oncotarget.27890
%U https://www.documentation.ird.fr/hor/fdi:010082444
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2021-08/010082444.pdf
%V 12
%W Horizon (IRD)
%X Hepatocellular carcinoma (HCC) usually afflicts individuals in their maturity after a protracted liver disease. Contrasting with this pattern, the age structure of HCC in Andean people displays a bimodal distribution with half of the patients developing HCC in adolescence and early adulthood. To deepen our understanding of the molecular determinants of the disease in this population, we conducted an integrative analysis of gene expression and DNA methylation in HCC developed by 74 Peruvian patients, including 39 adolescents and young adults. While genome-wide hypomethylation is considered as a paradigm in human HCCs, our analysis revealed that Peruvian tumors are associated with a global DNA hypermethylation. Moreover, pathway enrichment analysis of transcriptome data characterized an original combination of signatures. Peruvian HCC forgoes canonical activations of IGF2, Notch, Ras/MAPK, and TGF-? signals to depend instead on Hippo/YAP1, MYC, and Wnt/?-catenin pathways. These signatures delineate a homogeneous subtype of liver tumors at the interface of the proliferative and non-proliferative classes of HCCs. Remarkably, the development of this HCC subtype occurs in patients with one of the four Native American mitochondrial haplogroups A-D. Finally, integrative characterization revealed that Peruvian HCC is apparently controlled by the PRC2 complex that mediates cell reprogramming with massive DNA methylation modulating gene expression and pinpointed retinoid signaling as a potential target for epigenetic therapy.
%$ 020BIOCHI ; 050MEDECI ; 106ANTHRO1

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Vasquez-Ocmin, P. G.
%A Marti, G.
%A Bonhomme, M.
%A Mathis, F.
%A Fournier, S.
%A Bertani, Stéphane
%A Maciuk, A.
%T Cannabinoids vs. whole metabolome : relevance of cannabinomics in analyzing Cannabis varieties
%D 2021
%L fdi:010083800
%G ENG
%J Analytica Chimica Acta
%@ 0003-2670
%K Cannabis ; Cannabinomics ; Chemotypes ; Metabolome ; Phytocannabinoids
%M ISI:000724653500005
%P 339020 [12 ]
%R 10.1016/j.aca.2021.339020
%U https://www.documentation.ird.fr/hor/fdi:010083800
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2022-01/010083800.pdf
%V 1184
%W Horizon (IRD)
%X Cannabis sativa has a long history of domestication both for its bioactive compounds and its fibers. This has produced hundreds of varieties, usually characterized in the literature by chemotypes, with Delta(9)-THC and CBD content as the main markers. However, chemotyping could also be done based on minor compounds (phytocannabinoids and others). In this work, a workflow, which we propose to name cannabinomics, combines mass spectrometry of the whole metabolome and statistical analysis to help differentiate C. sativa varieties and deciphering their characteristic markers. By applying this cannabinomics approach to the data obtained from 20 varieties of C. sativa (classically classified as chemotype I, II, or III), we compared the results with those obtained by a targeted quantification of 11 phytocannabinoids. Cannabinomics can be considered as a complementary tool for phenotyping and genotyping, allowing the identification of minor compounds playing a key role as markers of differentiation.
%$ 076 ; 020

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Cerapio, J. P.
%A Perrier, M.
%A Pont, F.
%A Tosolini, M.
%A Laurent, C.
%A Bertani, Stéphane
%A Fournie, J. J.
%T Single-cell RNAseq profiling of human gamma delta T lymphocytes in virus-related cancers and COVID-19 disease
%D 2021
%L fdi:010083797
%G ENG
%J Viruses
%K human ; gammadelta ; lymphocyte ; tumor ; COVID-19 ; transcriptome ; single cell ; differentiation ; trajectory
%M ISI:000726850600001
%N 11
%P 2212 [14 ]
%R 10.3390/v13112212
%U https://www.documentation.ird.fr/hor/fdi:010083797
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2022-01/010083797.pdf
%V 13
%W Horizon (IRD)
%X The detailed characterization of human gamma delta T lymphocyte differentiation at the single-cell transcriptomic (scRNAseq) level in tumors and patients with coronavirus disease 2019 (COVID-19) requires both a reference differentiation trajectory of gamma delta T cells and a robust mapping method for additional gamma delta T lymphocytes. Here, we incepted such a method to characterize thousands of gamma delta T lymphocytes from (n = 95) patients with cancer or adult and pediatric COVID-19 disease. We found that cancer patients with human papillomavirus-positive head and neck squamous cell carcinoma and Epstein-Barr virus-positive Hodgkin's lymphoma have gamma delta tumor-infiltrating T lymphocytes that are more prone to recirculate from the tumor and avoid exhaustion. In COVID-19, both TCRV gamma 9 and TCRV gamma non9 subsets of gamma delta T lymphocytes relocalize from peripheral blood mononuclear cells (PBMC) to the infected lung tissue, where their advanced differentiation, tissue residency, and exhaustion reflect T cell activation. Although severe COVID-19 disease increases both recruitment and exhaustion of gamma delta T lymphocytes in infected lung lesions but not blood, the anti-IL6R therapy with Tocilizumab promotes gamma delta T lymphocyte differentiation in patients with COVID-19. PBMC from pediatric patients with acute COVID-19 disease display similar gamma delta T cell lymphopenia to that seen in adult patients. However, blood gamma delta T cells from children with the COVID-19-related multisystem inflammatory syndrome are not lymphodepleted, but they are differentiated as in healthy PBMC. These findings suggest that some virus-induced memory gamma delta T lymphocytes durably persist in the blood of adults and could subsequently infiltrate and recirculate in tumors.
%$ 050 ; 052 ; 020

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Cano, L.
%A Bertani, Stéphane
%A Island, M. L.
%A Cerapio, J. P.
%A Ruiz, E.
%A Pineau, P.
%A Monbet, V.
%A Boudjema, K.
%A Taxa, L.
%A Casavilca-Zambrano, S.
%A Ropert, M.
%A Turlin, B.
%A Loreal, O.
%T Metallomic profile in non-cirrhotic hepatocellular carcinoma supports a phenomenon of metal metabolism adaptation in tumor cells
%D 2021
%L fdi:010082679
%G ENG
%J Scientific Reports - Nature
%@ 2045-2322
%K PEROU ; FRANCE
%M ISI:000674490500002
%N 1
%P 14195 [10 ]
%R 10.1038/s41598-021-93369-4
%U https://www.documentation.ird.fr/hor/fdi:010082679
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2021-09/010082679.pdf
%V 11
%W Horizon (IRD)
%X We have previously described a form of hepatocellular carcinoma (HCC) in non-cirrhotic liver (HCC-NC) developed by Peruvian patients. We analyzed the metallomic profile in hepatic tissues from two independent cohorts exhibiting HCC-NC. Clinical, histopathological data, and HCC and non-tumoral liver (NTL) samples of 38 Peruvian and 38 French HCC-NC patients, were studied. Twelve metals were quantified using ICP/MS: Mn, Fe, Cu, Co, Zn, As, Se, Rb, Mo, Cd, Pb, and Sn. Associations between metals and survival were assessed. Our data showed significant differences between cohorts. Mean ages were 40.6 +/- 20, 67.5 +/- 9 years old for Peruvians and French, respectively. Fifty percent of the Peruvian patients were positive for the HBsAg, versus 3% in French patients. Mn, Cu, Zn, As, Se, Rb, Mo, Cd, Sn metal concentrations were higher in NTL of Peruvians. Importantly, metal concentrations were lower in HCC areas compared to NTL tissues in both cohorts, except for Cu for which mean concentration was higher in HCC (p<0.05). Se concentration in HCC was associated with extended survival only in Peruvians. Our data, obtained in Peruvian and French HCC-NC cohorts, highlights similarity in the metallomic profile of HCC compared to NTL during the hepatic tumorigenesis in these specific groups of patients.
%$ 050 ; 020

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Ducos, C.
%A Pinson-Gadais, L.
%A Chereau, S.
%A Richard-Forget, F.
%A Vasquez-Ocmin, P.
%A Cerapio, J. P.
%A Casavilca-Zambrano, S.
%A Ruiz, E.
%A Pineau, P.
%A Bertani, Stéphane
%A Ponts, N.
%T Natural occurrence of mycotoxin-producing fusaria in market-bought Peruvian cereals : a food safety threat for Andean populations
%D 2021
%L fdi:010081069
%G ENG
%J Toxins
%K Fumonisins B ; Peru ; corn
%K PEROU
%M ISI:000623295900001
%N 2
%P 172 [15 ]
%R 10.3390/toxins13020172
%U https://www.documentation.ird.fr/hor/fdi:010081069
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers21-03/010081069.pdf
%V 13
%W Horizon (IRD)
%X Consumption of cereals contaminated by mycotoxins poses health risks. For instance, Fumonisins B, mainly produced by Fusarium verticillioides and Fusarium proliferatum, and the type B trichothecene deoxynivalenol, typically produced by Fusarium graminearum, are highly prevalent on cereal grains that are staples of many cultural diets and known to represent a toxic risk hazard. In Peru, corn and other cereals are frequently consumed on a daily basis under various forms, the majority of food grains being sold through traditional markets for direct consumption. Here, we surveyed mycotoxin contents of market-bought grain samples in order to assess the threat these mycotoxins might represent to Peruvian population, with a focus on corn. We found that nearly one sample of Peruvian corn out of six was contaminated with very high levels of Fumonisins, levels mostly ascribed to the presence of F. verticillioides. Extensive profiling of Peruvian corn kernels for fungal contaminants could provide elements to refine the potential risk associated with Fusarium toxins and help define adapted food safety standards.
%$ 054 ; 050 ; 076 ; 020

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Casavilca-Zambrano, S.
%A Custodio, N.
%A Liendo-Picoaga, R.
%A Cancino-Maldonado, K.
%A Esenarro, L.
%A Montesinos, R.
%A Bertani, Stéphane
%A Fejerman, L.
%A Guerchet, M.
%A Vidaurre, T.
%T Depression in women with a diagnosis of breast cancer. Prevalence of symptoms of depression in Peruvian women with early breast cancer and related sociodemographic factors
%D 2020
%L fdi:010079998
%G ENG
%J Seminars in Oncology
%@ 0093-7754
%K Depression ; Breast cancer ; Psychiatric ; Beck depression inventory-II ; (BDI-II) ; Stages I and II breast cancer ; Early stage breast cancer
%K PEROU
%M ISI:000588327700006
%N 5
%P 293-301
%U https://www.documentation.ird.fr/hor/fdi:010079998
%> https://www.documentation.ird.fr/intranet/publi/2020/12/010079998.pdf
%V 47
%W Horizon (IRD)
%X We sought to review literature on the prevalence of symptoms of depression in women with a diagnosis of breast cancer (BC) and in the Peruvian population determine the prevalence of symptoms of depression and to describe the association with sociodemographic characteristics. Descriptive cross-sectional analytical study of 254 patients from the National Cancer Institute of Peru (Instituto Nacional de Enfermedades Neoplasicas) with a diagnosis of clinical stage I or II BC. The patients included women aged between 26 and 67 years old. Symptoms of depression were monitored by the Beck Depression Inventory-II. Moreover, clinical features and patient sociodemographic characteristics were analyzed and their association with depression was assessed by logistic regression. The average age of the patients was 47.8 +/- 9.2 years; 5.4% of the patients were postmenopausal at the time of the questionnaire. About 55% of women were from Lima, 58.3% had completed secondary education (11 +/- 3.2 years), 45.7% were not working, and 46.5% were single. The prevalence of depression was 25.6% at the time of BC diagnosis. Of those patients with symptoms of depression, 16.9% showed symptoms of mild depression, 6.3% moderate, and 2.4% severe. A multivariable logistic regression model showed that in Peruvian women with a diagnosis of BC being married or employed significantly decreased the odds of presenting depressive symptoms (P=0.029 and 0.017, respectively). Our main limitation was the lack of evaluation of depressive symptoms before the diagnosis, during or at the end of treatment. Another limitation was that the Beck Depression Inventory-II test could only identify depressive symptoms, but not depression as a disease. We have reviewed relevant literature on depression in women with a diagnosis of BC. The data presented suggests an association between both employment and marital status with depressive symptoms among Peruvian women with a diagnosis of BC. Pre-emptive support for women at risk could influence resilience and/or motivation for compliance with antineoplastic treatments.
%$ 050

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Pineau, P.
%A Ruiz, E.
%A Deharo, Eric
%A Bertani, Stéphane
%T On hepatocellular carcinoma in South America and early-age onset of the disease
%D 2019
%L fdi:010077041
%G ENG
%J Clinics and Research in Hepatology and Gastroenterology
%@ 2210-7401
%K Liver cancer ; Cancer risk factor ; Hepatitis B virus (HBV) ; Indigenous people ; Low- and middle-income countries ; Global health transition
%K PEROU ; ANDES
%M ISI:000488039200012
%N 5
%P 522-526
%R 10.1016/j.clinre.2018.10.019
%U https://www.documentation.ird.fr/hor/fdi:010077041
%> https://www.documentation.ird.fr/intranet/publi/2019/10/010077041.pdf
%V 43
%W Horizon (IRD)
%X Hepatocellular carcinoma (HCC) is one of the most predominant tumor types worldwide, being particularly prevalent in sub-Saharan Africa and East Asia. However, HCC is inexplicably underreported in South America, despite unsettling clinical epidemiological trends of the disease on this continent. Here, we review the current knowledge on HCC presentation in Peru. We emphasize the well-documented occurrence of an early-age nosological form of the disease in Andean descent populations. We further discuss the reasons for such HCC clinical presentation, as well as the implications for liver cancer screening, management, and prevention.
%$ 050 ; 052

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Marchio, A.
%A Cerapio, J. P.
%A Ruiz, E.
%A Cano, L.
%A Casavilca, S.
%A Terris, B.
%A Deharo, Eric
%A Dejean, A.
%A Bertani, Stéphane
%A Pineau, P.
%T Early-onset liver cancer in South America associates with low hepatitis B virus DNA burden
%D 2018
%L fdi:010075252
%G ENG
%J Scientific Reports - Nature
%@ 2045-2322
%K PEROU
%M ISI:000460206300001
%P art. 12031 [14 ]
%R 10.1038/s41598-018-30229-8
%U https://www.documentation.ird.fr/hor/fdi:010075252
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers19-03/010075252.pdf
%V 8
%W Horizon (IRD)
%X In Peru, hepatocellular carcinoma (HCC) arises in young non-cirrhotic patients. Hepatitis B virus (HBV) is suspected to be the prominent etiological agent. We thus performed a comprehensive molecular study of HBV infection in 65 Peruvian HCC patients. Only 51% were considered as persistently infected at the onset. HBV DNA was found by PCR in the tumor and/or matched non-tumor liver tissues in more than 80% of cases (n = 53/65). HBV DNA was significantly more abundant in livers of younger patients than in those of the older ones. We consistently observed low viral DNA burden (0.1-6.5 copies for 100 cells), with viral genomes in younger patients displaying higher proportion of mutations at di-pyrimidines (TpT and CpC, P = 0.006). A drastic activation of multiple DNA repair pathways in tumors of younger patients was observed. Our observations clearly challenge the current vision that associates high HBV DNA load with earlier tumor development. We concluded that in Peru, and maybe in other populations with Americas' indigenous ancestry, HBV-associated liver tumorigenesis might differ significantly from that generally observed in the rest of the world. Procedures used to screen for HCC development in subjects at risk should be adapted to the local situation.
%$ 052 ; 050 ; 020

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Paboriboune, P.
%A Vial, Thomas
%A Sitbounlang, P.
%A Bertani, Stéphane
%A Trepo, C.
%A Deny, P.
%A Babin, F. X.
%A Steenkeste, N.
%A Pineau, P.
%A Deharo, Eric
%T Hepatitis C in Laos : a 7-year retrospective study on 1765 patients
%D 2018
%L fdi:010073995
%G ENG
%J Virologica Sinica
%@ 1674-0769
%K Hepatitis C virus (HCV) ; HCV incidence ; Antiviral agents ; Laos
%K LAOS
%M ISI:000444001200001
%N 4
%P 295-303
%R 10.1007/s12250-018-0039-9
%U https://www.documentation.ird.fr/hor/fdi:010073995
%> https://www.documentation.ird.fr/intranet/publi/2018/09/010073995.pdf
%V 33
%W Horizon (IRD)
%X Hepatitis C virus (HCV) is a global health concern, notably in Southeast Asia, and in Laos the presentation of the HCV-induced liver disease is poorly known. Our objective was thus to describe a comprehensive HCV infection pattern in order to guide national health policies. A study on a group of 1765 patients formerly diagnosed by rapid test in health centres was conducted at the Centre of Infectiology Lao Christophe Merieux in Vientiane. The demographic information of patients, their infection status (viral load: VL), liver function (aminotransferases) and treatments were analysed. Results showed that gender distribution of infected people was balanced; with median ages of 53.8 for men and 51.6years for women (13-86years). The majority of patients (72%) were confirmed positive (VL>50IU/mL) and 28% of them had high VL (>6log(10)). About 23% of patients had level of aminotransferases indicative of liver damage (>40IU/mL); but less than 20% of patients received treatment. Patients rarely received a second sampling or medical imaging. The survey also showed that cycloferon, pegylated interferon and ribavirin were the drugs prescribed preferentially by the medical staff, without following any international recommendations schemes. In conclusion, we recommend that a population screening policy and better management of patients should be urgently implemented in the country, respecting official guidelines. However, the cost of biological analysis and treatment are significant barriers that must be removed. Public health resolutions should be immediately enforced in the perspective of meeting the WHO HCV elimination deadline by 2030.
%$ 052 ; 050

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Cano, L.
%A Cerapio, J. P.
%A Ruiz, E.
%A Marchio, A.
%A Turlin, B.
%A Casavilca, S.
%A Taxa, L.
%A Marti, G.
%A Deharo, Eric
%A Pineau, P.
%A Bertani, Stéphane
%T Liver clear cell foci and viral infection are associated with non-cirrhotic, non-fibrolamellar hepatocellular carcinoma in young patients from South America
%D 2018
%L fdi:010073707
%G ENG
%J Scientific Reports - Nature
%@ 2045-2322
%K PEROU
%M ISI:000440145900001
%P art. 9945 [12 ]
%R 10.1038/s41598-018-28286-0
%U https://www.documentation.ird.fr/hor/fdi:010073707
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers18-09/010073707.pdf
%V 8
%W Horizon (IRD)
%X We previously described a divergent clinical and molecular presentation of hepatocellular carcinoma (HCC) in Peru. The present study aimed to further characterize the tissue features associated with this singular nosological form of HCC in order to gain insight into the natural history of the disease. We performed an exploratory analysis of the histology of both tumor and non-tumor liver (NTL) tissues from 50 Peruvian HCC patients, and compared with that of 75 individuals with non-HCC liver tumor or benign liver lesions as a baseline for NTL features. We complemented this approach with a transcriptome analysis in a subset of NTL tissue samples and also performed an ultra-sensitive hepatitis B virus (HBV) detection in liver tissues of the patients. Overall, results highlighted the low rate of liver parenchymal alterations in a young patient cohort (median age: 40 years old), despite a strong prevalence of underlying HBV infection (c. 67%). Withal, liver clear cell foci of cellular alteration were genuinely associated with HCC and appended to some changes in immune and G protein-coupled receptor gene expression ontologies. Our findings confirm the occurrence of a particular setting of HCC in South America, a region where the pathophysiology of liver cancer remains largely unexplored.
%$ 050 ; 052

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Machicado, C.
%A Bertani, Stéphane
%A Herrera-Velit, P.
%A Espinoza, J.
%A Ruiz, E.
%A Marcos, L.
%T Negative serology of Fasciola hepatica infection in patients with liver cancer in Peru : a preliminary report
%D 2018
%L fdi:010073026
%G ENG
%J Revista da Sociedade Brasileira de Medicina Tropical
%@ 0037-8682
%K Cholangiocarcinoma ; Fasciolosis ; Fibrosis ; Hepatocellular carcinoma ; Liver fluke
%K PEROU
%M ISI:000432390600019
%N 2
%P 231-233
%R 10.1590/0037-8682-0180-2017
%U https://www.documentation.ird.fr/hor/fdi:010073026
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers18-06/010073026.pdf
%V 51
%W Horizon (IRD)
%X Introduction: The etiology of several hepatocellular carcinoma (HCC) cases remains largely unknown. Although Fasciola hepatica has been associated with liver fibrosis in Latin America, it has not yet been associated with HCC. This study aimed to determine the existence of specific IgG antibodies against F. hepatica in the serum samples of HCC patients. Methods: In total, 13 scrum samples from 13 HCC patients were screened using Fas2-ELISA. Results: Fas2-ELISA demonstrated negative results in all HCC patients included in this study. Conclusions: The pre-existence of F hepatica infection in HCC patients needs to be further investigated in epidemiological and experimental studies.
%$ 052 ; 050

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Paboriboune, P.
%A Vial, Thomas
%A Chassagne, F.
%A Sitbounlang, P.
%A Soundala, S.
%A Bertani, Stéphane
%A Sengmanothong, D.
%A Babin, F. X.
%A Steenkeste, N.
%A Deny, P.
%A Pineau, P.
%A Deharo, Eric
%T A seven-year retrospective study on the surveillance of hepatitis B in Laos
%D 2018
%L fdi:010072740
%G ENG
%J International Journal of Hepatology
%@ 2090-3448
%K LAOS
%M ISI:000430270700001
%P art. ID 9462475 [11 ]
%R 10.1155/2018/9462475
%U https://www.documentation.ird.fr/hor/fdi:010072740
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers18-05/010072740.pdf
%W Horizon (IRD)
%X Objective. Lao PDR is one of the most highly endemic countries for hepatitis B in Asia and the second country for liver cancer incidence. Therefore, the follow-up of infected individuals through predictive serological markers is of utmost importance to monitor the progression of the pathology and take the decision on treatment. Methods. A retrospective-descriptive cohort study was conducted on 3,857 HBV-infected patients. Information about infection status (viral load, VL), liver function (aminotransferases), and treatments was recorded. Results. M/F sex ratio was 1.77 for a median age of 37. Patients under 37 displayed higher VL than older ones and men had higher VL than women. Initial VL ranged from <50 IU/mL to 2.5 10(13) IU/mL. Median aminotransferase values were 45.5 U/L for ALAT and 44 U/L for ASAT, ranging from <8 to >2,000 U/L. Men had higher aminotransferase than women. Globally 20% of patients received treatment (mainly immunostimulant and reverse-transcriptase inhibitors); 11% had high levels of VL and liver enzymes, but only 2% of them were treated. Conclusion. Public health decisions should be taken urgently to rationalise vaccination and provide fair access to early diagnosis and treatment; otherwise the burden of HBV-associated diseases will be overwhelming for Laos in the near future.
%$ 050 ; 052

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Rojas, T. R.
%A Bourdy, Geneviève
%A Ruiz, E.
%A Cerapio, J. P.
%A Pineau, P.
%A Gardon, Jacques
%A Doimi, F.
%A Deparis, X.
%A Deharo, Eric
%A Bertani, Stéphane
%T Herbal medicine practices of patients with liver cancer in Peru : a comprehensive study toward integrative cancer management
%D 2018
%L fdi:010072370
%G ENG
%J Integrative Cancer Therapies
%@ 1534-7354
%K behavioral epidemiological survey ; cancer ; complementary and alternative medicine ; developing world ; ethnobotany ; Indigenous people ; integrative medicine ; Latin America ; liver disease ; traditional medicine
%K PEROU
%M ISI:000425156600006
%N 1
%P 52-64
%R 10.1177/1534735416681642
%U https://www.documentation.ird.fr/hor/fdi:010072370
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers18-03/010072370.pdf
%V 17
%W Horizon (IRD)
%X Rationale: The highest burden of liver cancer occurs in developing countries, where the use of herbal medicine (HM) is still widespread. Despite this trend, few studies have been conducted to report HM practices of patients with a hepatic tumor in the developing world. Hence, this study aimed to document the use of HM among patients with liver cancer in Peru. Study Design and Methods: A comparative behavioral epidemiological survey was conducted among liver cancer patients attending the National Cancer Institute of Peru. Information was obtained by direct interviews based on a semistructured questionnaire. The use of HM in Peruvian liver cancer patients was reported, first, regarding general consumption prior to the onset of disease, and second, after the appearance of symptoms that patients would relate to their tumor. In parallel, general consumption of HM in noncancerous people was assessed as a comparative figure. A correspondence analysis was performed to reveal potential associations between the symptoms of cancer and the specific use of HM. Results: Eighty-eight patients and 117 noncancerous individuals participated in the survey. Overall, 68.3% of the people interviewed claimed to use HM on a regular basis for general health preservation. Furthermore, 56.8% of the patients turned to plants first to treat the disorders for which they later came to the cancer care center. When compared with the number of plant species used routinely (n = 78), a selection of plants was made by patients in response to the symptoms of cancer (n = 46). At least 2 plant species, Aloe vera and Morinda citrifolia, were significantly associated with the treatment of liver cancer-related symptoms in the patient group. Conclusions: The present study is the first survey on the HM practices of patients with liver cancer in Latin America and, more broadly, in the developing world. Our findings confirm that HM remains one of the principal primary health care resources in Peru, even for a severe disease like liver cancer. These traditional, complementary and alternative medicine practices should be taken into consideration in Peruvian health programs aiming to educate the population in cancer prevention and treatment, as well as integrative cancer management.
%$ 076 ; 050

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Ruiz, E.
%A Rojas Rojas, T.
%A Berrospi, F.
%A Chávez, I.
%A Luque, C.
%A Cano, L.
%A Doimi, F.
%A Pineau, P.
%A Deharo, Eric
%A Bertani, Stéphane
%T Hepatocellular carcinoma surgery outcomes in the developing world : a 20-year retrospective cohort study at the National Cancer Institute of Peru
%D 2016
%L fdi:010070745
%G ENG
%J Heliyon
%@ 2405-8440
%K PEROU
%N 1
%P art. e00052 [19  en ligne]
%R 10.1016/j.heliyon.2015.e00052
%U https://www.documentation.ird.fr/hor/fdi:010070745
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-08/010070745.pdf
%V 2
%W Horizon (IRD)
%$ 052 ; 050

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Chassagne, F.
%A Rojas, T. R.
%A Bertani, Stéphane
%A Bourdy, Geneviève
%A Eav, S.
%A Ruiz, E.
%A Pineau, P.
%A Deharo, Eric
%T A 13-year retrospective study on primary liver cancer in Cambodia : a strikingly high hepatitis C occurrence among hepatocellular carcinoma cases
%D 2016
%L fdi:010067735
%G ENG
%J Oncology
%@ 0030-2414
%K Hepatocellular carcinoma ; Cholangiocarcinoma ; Hepatitis B virus ; Hepatitis C virus ; Opisthorchis viverrini ; Cambodia
%K CAMBODGE
%M ISI:000381655900007
%N 2
%P 106-116
%R 10.1159/000446398
%U https://www.documentation.ird.fr/hor/fdi:010067735
%> https://www.documentation.ird.fr/intranet/publi/2016/09/010067735.pdf
%V 91
%W Horizon (IRD)
%X Objectives: Hepatocellular carcinoma (HCC) is the main type of primary liver cancer (PLC) worldwide, but cholangio-carcinoma (CCA) may be predominant in some specific regions of Southeast Asia. The aim of the present study was to delineate a pattern of Cambodian PLC patients attending the Calmette Hospital in the Cambodian capital Phnom Penh. Materials and Methods: A total of 553 medical charts diagnosing PLCs from January 2003 to May 2015 were obtained from both the Oncology and Hepato-Gastroenterology Departments of the Calmette Hospital. Results: HCC was the predominant type of PLC recorded, with 511 cases (92.4%), whereas CCA represented merely 7.6% (42 cases) of the overall series. Hepatitis B virus (HBV; 44.3%) and hepatitis C virus (HCV; 43%) infection rates were similar among the HCC patients, while small subsets of CCA patients were infected with HBV (15.4%) or HCV (11.5%). Most HCC (84%) and CCA (73.8%) patients received palliative treatment only. Conclusion: The present study indicates that HCC is the main form of primary hepatic neoplasm among PLC patients attending a hospital in Cambodia. HBV and HCV infections represented equivalent burdens and major contributing factors to HCC. Therefore, the implementation of prevention programs for these infectious agents should become a priority for health policy makers in the country.
%$ 052 ; 050

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Marchio, A.
%A Bertani, Stéphane
%A Rojas, T. R.
%A Doimi, F.
%A Terris, B.
%A Deharo, Eric
%A Dejean, A.
%A Ruiz, E.
%A Pineau, P.
%T A peculiar mutation spectrum emerging from young Peruvian patients with hepatocellular carcinoma
%D 2014
%L fdi:010063661
%G ENG
%J Plos One
%@ 1932-6203
%K PEROU
%M ISI:000347146700070
%N 12
%P e114912 [24 ]
%R 10.1371/journal.pone.0114912
%U https://www.documentation.ird.fr/hor/fdi:010063661
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-10/010063661.pdf
%V 9
%W Horizon (IRD)
%X Hepatocellular carcinoma usually afflicts individuals in their later years following longstanding liver disease. In Peru, hepatocellular carcinoma exists in a unique clinical presentation, which affects patients around age 25 with a normal, healthy liver. In order to deepen our understanding of the molecular processes ongoing in Peruvian liver tumors, mutation spectrum analysis was carried out on hepatocellular carcinomas from 80 Peruvian patients. Sequencing analysis focused on nine genes typically altered during liver carcinogenesis, i.e. ARID2, AXIN1, BRAF, CTNNB1, NFE2L2, H/K/N-RAS, and TP53. We also assessed the transcription level of factors involved in the control of the alpha-fetoprotein expression and the Hippo signaling pathway that controls contact inhibition in metazoans. The mutation spectrum of Peruvian patients was unique with a major class of alterations represented by Insertions/Deletions. There were no changes at hepatocellular carcinoma-associated mutation hotspots in more than half of the specimens analyzed. Furthermore, our findings support the theory of a consistent collapse in the Hippo axis, as well as an expression of the stemness factor NANOG in high alpha-fetoprotein-expressing hepatocellular carcinomas. These results confirm the specificity of Peruvian hepatocellular carcinoma at the molecular genetic level. The present study emphasizes the necessity to widen cancer research to include historically neglected patients from South America, and more broadly the Global South, where cancer genetics and tumor presentation are divergent from canonical neoplasms.
%$ 050

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Deharo, Eric
%A Jullian, Valérie
%A Bertani, Stéphane
%A Bourdy, Geneviève
%T La simalikalactone E, molécule antipaludique et anticancer = Simalikalactone E, anti-malarial and anticancer molecule
%D 2014
%L fdi:010061732
%G FRE
%J Biofutur
%@ 0294-3506
%K AMAZONIE
%M ISI:000329587500059
%N 350
%P 56-59
%U https://www.documentation.ird.fr/hor/fdi:010061732
%> https://www.documentation.ird.fr/intranet/publi/2014/02/010061732.pdf
%W Horizon (IRD)
%X En octobre dernier, le Prix 2013 de l’innovation pour le Sud décerné par l’Institut de recherche pour le développement (IRD) récompensait la découverte d’une molécule aux propriétés miraculeuses : la simalikalactone E. Isolé de Quassia amara, une plante médicinale amazonienne, cette molécule est à la fois un antipaludique et un anticancer. Cette découverte permettra-t-elle à la plante d’accéder au statut des grandes plantes médicaments qui ont bouleversé la thérapeutique en sauvant des milliers de vies ? C’est l’espoir nourri par les chercheurs. Histoire d’une découverte.
%$ 076 ; 020

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Bertani, Stéphane
%A Pineau, P.
%A Loli, S.
%A Moura, Julien
%A Zimic, M.
%A Deharo, Eric
%A Ruiz, E.
%T An atypical age-specific pattern of hepatocellular carcinoma in Peru : a threat for Andean populations
%D 2013
%L fdi:010060508
%G ENG
%J Plos One
%@ 1932-6203
%K PEROU ; ANDES
%M ISI:000321148400132
%N 6
%P e67756
%R 10.1371/journal.pone.0067756
%U https://www.documentation.ird.fr/hor/fdi:010060508
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-10/010060508.pdf
%V 8
%W Horizon (IRD)
%X Background: In South America, the highest incidence of primary liver cancer is observed in Peru. However, national estimations on hepatocellular carcinoma incidence and mortality are approximated using aggregated data from surrounding countries. Thus, there is a lack of tangible information from Peru that impairs an accurate description of the local incidence, presentation, and outcomes of hepatocellular carcinoma. The present study attempts to fill this gap and assesses the clinical epidemiology of hepatocellular carcinoma in this country. Methods: A retrospective cohort study was conducted by analysing the medical charts of 1,541 patients with hepatocellular carcinoma admitted between 1997 and 2010 at the Peruvian national institute for cancer. The medical records including liver function, serologic status, and tumor pathology and stage were monitored. Statistical analyses were performed in order to characterize tumor presentation according to demographic features, risk factors, and regional origin. Results: Surprisingly, the age distribution of the patient population displayed bimodality corresponding to two distinct age-based subpopulations. While an older group was in keeping with the age range observed for hepatocellular carcinoma around the world, a younger population displayed an abnormally juvenile mean age of 25.5 years old. In addition, each subpopulation displayed age-specific pathophysiological and clinical characteristics. Conclusions: The analysis suggests two different age-specific natural histories of hepatocellular carcinoma in the Peruvian patient population. This otherwise unusual tumor process that is ongoing in younger patients leads to the hypothesis that there may be a Peru-endemic risk factor driving hepatocarcinogenesis in the local population.
%$ 050

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Garavito, G.
%A Bertani, Stéphane
%A Quiliano, M.
%A Valentin, A.
%A Aldana, I.
%A Deharo, Eric
%T The in vivo antimalarial activity of methylene blue combined with pyrimethamine, chloroquine and quinine
%D 2012
%L fdi:010057237
%G ENG
%J Memorias do Instituto Oswaldo Cruz
%@ 0074-0276
%K antimalarials ; methylene blue ; drug combination
%M ISI:000308943900019
%N 6
%P 820-823
%U https://www.documentation.ird.fr/hor/fdi:010057237
%> https://www.documentation.ird.fr/intranet/publi/2012/10/010057237.pdf
%V 107
%W Horizon (IRD)
%X The effectiveness of methylene blue (MB) combined with pyrimethamine (PYR), chloroquine (CQ) or quinine (Q) was examined in a classical four-day suppressive test against a causative agent of rodent malaria, Plasmodium berghei. A marked potentiation was observed when MB was administered at a non-curative dose of 15 mg/kg/day in combination with PYR (0.19 mg/kg/day) or Q (25 mg/kg/day). No synergy was found between MB (15 mg/Kg) and CQ (0.75 mg/Kg). Our results suggest that the combination of MB with PYR or Q may improve the efficacy of these currently used antimalarial drugs.
%$ 052

%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Bertani, Stéphane
%A Houel, E.
%A Jullian, Valérie
%A Bourdy, Geneviève
%A Valentin, A.
%A Stien, D.
%A Deharo, Eric
%T New findings on Simalikalactone D, an antimalarial compound from Quassia amara L. (Simaroubaceae)
%D 2012
%L fdi:010055854
%G ENG
%J Experimental Parasitology
%@ 0014-4894
%K Antimalarial ; Simalikalactone D ; Quassinoid ; Plasmodium ; Quassia amara
%K PEROU
%M ISI:000303081500005
%N 4
%P 341-347
%R 10.1016/j.exppara.2012.02.013
%U https://www.documentation.ird.fr/hor/fdi:010055854
%> https://www.documentation.ird.fr/intranet/publi/2012/05/010055854.pdf
%V 130
%W Horizon (IRD)
%X Quassia amara L (Simaroubaceae) is a species widely used as tonic and is claimed to be an efficient antimalarial all over the Northern part of the Amazon basin. Quassinoid compound Simalikalactone D (SkD) has been shown to be one of the molecules responsible for the antiplasmodial activity of a watery preparation made out of juvenile fresh leaves of this plant. Because of its strong antimalarial activity, we decided to have a further insight of SkD pharmacological properties, alone or in association with classical antimalarials. At concentrations of up to 200 mu M, we showed herein that SkD did not exert any apoptotic or necrotic activities in vitro on lymphoblastic cells. However, an antiproliferative effect was evident at concentrations higher than 45 nM. SkD was inefficient at inhibiting heme biomineralization and the new permeability pathways induced by the parasite in the host erythrocyte membrane. With respect to Plasmodium falciparum erythrocytic stages, SkD was almost inactive on earlier and later parasite stages, but potently active at the 30th h of parasite cycle when DNA replicates in mature trophozoites. In vitro combination studies with conventional antimalarial drugs showed that SkD synergizes with atovaquone (ATO). The activity of ATO on the Plasmodium mitochondrial membrane potential was enhanced by SkD, which on its own had a poor effect on this cellular parameter.
%$ 076 ; 052