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      <ref-type name="Journal Article">17</ref-type>
      <work-type>ACLN : Articles dans des revues avec comité de lecture non répertoriées par l'AERES</work-type>
      <contributors>
        <authors>
          <author>
            <style face="bold" font="default" size="100%">Roussos, Sevastianos</style>
          </author>
          <author>
            <style face="bold" font="default" size="100%">Raimbault, Maurice</style>
          </author>
          <author>
            <style face="normal" font="default" size="100%">Saucedo-Castaneda, G.</style>
          </author>
          <author>
            <style face="normal" font="default" size="100%">Lonsane, B.K.</style>
          </author>
        </authors>
      </contributors>
      <titles>
        <title>Efficient leaching of cellulases produced by Trichoderma harzianum in solid state fermentation</title>
        <secondary-title>Biotechnology Techniques</secondary-title>
      </titles>
      <pages>429-432</pages>
      <keywords>
        <keyword>BIOTECHNOLOGIE</keyword>
        <keyword>FERMENTATION EN MILIEU SOLIDE</keyword>
        <keyword>MICROORGANISME</keyword>
        <keyword>EXTRACTION</keyword>
        <keyword>CELLULASE</keyword>
        <keyword>ACTIVITE ENZYMATIQUE</keyword>
        <keyword>CANNE A SUCRE</keyword>
        <keyword>BAGASSE</keyword>
        <keyword>METHODE</keyword>
        <keyword>PHASE LIQUIDE</keyword>
        <keyword>CHAMPIGNON FILAMENTEUX</keyword>
      </keywords>
      <dates>
        <year>1992</year>
      </dates>
      <call-num>fdi:39185</call-num>
      <language>ENG</language>
      <periodical>
        <full-title>Biotechnology Techniques</full-title>
      </periodical>
      <isbn>0951-208X</isbn>
      <number>5</number>
      <electronic-resource-num>10.1007/BF02447483</electronic-resource-num>
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          <url>https://www.documentation.ird.fr/hor/fdi:39185</url>
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          <url>https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_33-34/39185.pdf</url>
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      </urls>
      <volume>6</volume>
      <remote-database-provider>Horizon (IRD)</remote-database-provider>
      <abstract>Recovery of cellulases from solid state culture of #Trichoderma harzianum$ was efficiently achieved by hydraulic pressing. Pressing of fermented solids yielded carboxymethyl-cellulase (CMCase) extraction efficiency of 71% and a ratio of leachate to fermented solids of 0.58 (v/w). Addition of water to pressed solids and second pressing improved the efficiency (95%) with simultaneous increase in the ratio to 1.16 (v/w). The overall extraction of filter paper activity was lower (85%) than that of CMCase. This technique is simple and its extraction efficiency is similar to that obtained in multiple-contact counter-current systems. The hydraulic press in its individuality was not used earlier to leach the product from fermented solids. (Résumé d'auteur)</abstract>
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