Antier Philip auteur aut IRD Minjares A. auteur aut IRD Roussos Sevastianos auteur aut IRD Raimbault Maurice auteur aut IRD Viniegra-Gonzalez G. auteur aut IRD text journalArticle eng text/pdf reformatted digital access The aim of this study was to improve mold strains for the production of pectinases by solid-state fermentation (SSF) of coffee pulp. A screening of 248 strains, isolated in Mexico's coffee-growing areas, permitted us to select a wild strain of Aspergillus niger which in 72 h attains a peak production of 27.7 U ml-1(138 U g-1 dry pulp) of pectinase measured by viscosimetry. Though the use of a selective culture medium with low water activity (aw = 0.954) with 2-deoxy-glucose (2DG) it was possible to isolate pectinase-hyperproductive mutant strains for SSF(44.5 U ml-1, 228 U g-1 dry pulp). Derepressed mutant strains that hyperproduced pectinase by submerged fermentation (SmF) were also obtained using a classic selective medium with high water activity (pectin + 2DG) with aw = 0.999. A comparison between both classes of mutants, called AW96 and AW99, respectively, points out the need to design special selective media in order to obtain strains adapted either to SSF or Smf in which the aw level would be a key selecting factor. (Résumé d'auteur) specialized FERMENTATION EN MILIEU SOLIDE PULPE CAFE ENZYME CHAMPIGNON ETUDE COMPARATIVE ISOLEMENT SOUCHE MILIEU DE CULTURE ACTIVITE ENZYMATIQUE MUTANT PECTINASE CARACTERISATION 084FERMEN 15 254-260 1993 0141-0229 https://www.documentation.ird.fr/hor/fdi:39182 10.1016/0141-0229(93)90146-S 0141-0229 [F B39182] https://www.documentation.ird.fr/hor/fdi:39182 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_33-34/39182.pdf IRD - Base Horizon / Pleins textes 1994-03-29 2006-06-06 fdi:39182 fre