Esterases of the flounder (#Platichthys flesus$, #Pleuronectidae$, #Teleostei$) : development of an identification protocol using starch gel electrophoresis and characterization of loci

Esterases of the flounder (#Platichthys flesus$, #Pleuronectidae$, #Teleostei$) : development of an identification protocol using starch gel electrophoresis and characterization of loci Berrebi P. auteur aut IRD Landaud P. auteur aut IRD Borsa P. auteur aut IRD Renno Jean-François auteur aut IRD text journalArticle eng text/pdf reformatted digital access Fish esterases are among the most difficult enzymes to identify using starch gel electrophoresis because of the many loci that are simultaneously active, and especially because of duplication phenomena, satellite bands, and stain trails. In an attempt to simplify and clarify electropherograms, various staining and inhibitory methods were tested on esterases from the flounder Platichthys flesus. A range of migration and staining buffers and substrates were used, as well as chemical inhibition and heat inactivation. A combination of the methods made it possible to distinguish and characterize the five presumed esterase loci. (Résumé d'auteur) specialized POISSON D'EAU DOUCE GENETIQUE DE POPULATION ENZYME INHIBITION ELECTROPHORESE 080ZOOGEN03 020BIOL Experientia 46 863-867 1990 0014-4754 https://www.documentation.ird.fr/hor/fdi:36715 10.1007/BF01935540 0014-4754 [F B36715] https://www.documentation.ird.fr/hor/fdi:36715 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_33-34/36715.pdf IRD - Base Horizon / Pleins textes 1993-02-09 2006-06-06 fdi:36715 fre