%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Duhoux, E.
%A Sougoufara, B.
%A Dommergues, Yvon
%T Propagation of Casuarina equisetifolia through axillary buds of immature female inflorescences cultured in vitro
%D 1986
%L fdi:23227
%G ENG
%J Plant Cell Reports
%@ 0721-7714
%K SYMBIOSE ; BOURGEON ; CULTURE IN VITRO ; MILIEU DE CULTURE ; PLANTULE ; SOL ; METHODOLOGIE
%K ACTINORHIZE ; AXILLAIRE ; MICROPROPAGATION ; INFLORESCENCE FEMELLE IMMATURE ; EXPLANT
%P 161-164
%R 10.1007/BF00269108
%U https://www.documentation.ird.fr/hor/fdi:23227
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_5/b_fdi_18-19/23227.pdf
%V 3
%W Horizon (IRD)
%X The study of the actinorhizal symbiosis in #Casuarina equisetifolia$ requires an homogenous plant material. Consequently, we devised a method of micropropagation based on the use of immature female inflorescences (IFI) as explants. IFI excised from an adult tree formed multiple buds after 4-week incubation on Murashige and Skoog medium with 0.05 micromol 1-1 NAA and 11.1 micromol 1-1 BAP. The axillary buds evolved into 5-6 cm long shoots 5 weeks after the transfer of IFI on a similar medium except for the addition of activated charcoal. Rooting of the shoots was obtained on a third medium, without BAP or charcoal, but with 1 micromol 1-1 NAA. The plantlets were transferred into soil. Their growth was satisfactory and no plagiotropic tendency was observed. (Résumé d'auteur)
%$ 084VITRO