@article{fdi:23227, title = {{P}ropagation of {C}asuarina equisetifolia through axillary buds of immature female inflorescences cultured in vitro}, author = {{D}uhoux, {E}. and {S}ougoufara, {B}. and {D}ommergues, {Y}von}, editor = {}, language = {{ENG}}, abstract = {{T}he study of the actinorhizal symbiosis in #{C}asuarina equisetifolia$ requires an homogenous plant material. {C}onsequently, we devised a method of micropropagation based on the use of immature female inflorescences ({IFI}) as explants. {IFI} excised from an adult tree formed multiple buds after 4-week incubation on {M}urashige and {S}koog medium with 0.05 micromol 1-1 {NAA} and 11.1 micromol 1-1 {BAP}. {T}he axillary buds evolved into 5-6 cm long shoots 5 weeks after the transfer of {IFI} on a similar medium except for the addition of activated charcoal. {R}ooting of the shoots was obtained on a third medium, without {BAP} or charcoal, but with 1 micromol 1-1 {NAA}. {T}he plantlets were transferred into soil. {T}heir growth was satisfactory and no plagiotropic tendency was observed. ({R}{\'e}sum{\'e} d'auteur)}, keywords = {{SYMBIOSE} ; {BOURGEON} ; {CULTURE} {IN} {VITRO} ; {MILIEU} {DE} {CULTURE} ; {PLANTULE} ; {SOL} ; {METHODOLOGIE} ; {ACTINORHIZE} ; {AXILLAIRE} ; {MICROPROPAGATION} ; {INFLORESCENCE} {FEMELLE} {IMMATURE} ; {EXPLANT}}, booktitle = {}, journal = {{P}lant {C}ell {R}eports}, volume = {3}, numero = {}, pages = {161--164}, ISSN = {0721-7714}, year = {1986}, DOI = {10.1007/{BF}00269108}, URL = {https://www.documentation.ird.fr/hor/fdi:23227}, }