Duhoux E., Sougoufara B., Dommergues Yvon. (1986). Propagation of Casuarina equisetifolia through axillary buds of immature female inflorescences cultured in vitro. Plant Cell Reports, 3, p. 161-164. ISSN 0721-7714.
Titre du document
Propagation of Casuarina equisetifolia through axillary buds of immature female inflorescences cultured in vitro
Année de publication
1986
Type de document
Article
Auteurs
Duhoux E., Sougoufara B., Dommergues Yvon
Source
Plant Cell Reports, 1986,
3, p. 161-164 ISSN 0721-7714
The study of the actinorhizal symbiosis in Casuarina equisetifolia requires an homogenous plant material. Consequently, we devised a method of micropropagation based on the use of immature female inflorescences (IFI) as explants. IFI excised from an adult tree formed multiple buds after 4-week incubation on Murashige and Skoog medium with 0.05 micromol 1-1 NAA and 11.1 micromol 1-1 BAP. The axillary buds evolved into 5-6 cm long shoots 5 weeks after the transfer of IFI on a similar medium except for the addition of activated charcoal. Rooting of the shoots was obtained on a third medium, without BAP or charcoal, but with 1 micromol 1-1 NAA. The plantlets were transferred into soil. Their growth was satisfactory and no plagiotropic tendency was observed. (Résumé d'auteur)
Plan de classement
Culture végétale in vitro [084VITRO]
Descripteurs
SYMBIOSE ; BOURGEON ; CULTURE IN VITRO ; MILIEU DE CULTURE ; PLANTULE ; SOL ; METHODOLOGIE ; ACTINORHIZE ; AXILLAIRE ; MICROPROPAGATION ; INFLORESCENCE FEMELLE IMMATURE ; EXPLANT
