@article{fdi:010097348,
  title = {{U}nlocking fine-scale temporal dynamics of coral reef health : a multi-kingdom microbial fingerprinting approach across seawater and sediment},
  author = {{S}tenger, {P}. {L}. and {T}ribollet, {A}line and {C}uet, {P}. and {G}uilhaumon, {F}ran{\c{c}}ois and {M}angion, {P}. and {B}ureau, {S}. and {F}erreira, {S}. and {P}ennober, {G}. and {J}ourand, {P}hilippe},
  editor = {},
  language = {{ENG}},
  abstract = {{C}oral reefs are rapidly degrading worldwide, creating a need for sensitive biomonitoring tools to detect early ecological shifts before visible collapse. {T}raditional monitoring often misses these incipient changes. {W}e hypothesized that multi-kingdom microbial fingerprinting could reveal fine-scale temporal dynamics and early bioindicators of reef environmental change. {T}he study was conducted on the fringing reef of {R}eunion {I}sland (south-west {I}ndian {O}cean), a system exposed to local anthropogenic pressures. {S}eawater and sediment samples were collected bimonthly from {A}pril to {O}ctober 2022 at three reef sites. {B}acterial (16{S} r{RNA}), fungal ({ITS}2), microalgal ({T}uf{A}), and protistal (18{S} r{RNA}) communities were characterized using metabarcoding. {P}hysicochemical parameters were integrated via multivariate analyses. {T}ime emerged as a stronger structuring factor than space. {B}ayesian analyses identified 368 temporally associated {ASV}s ({A}mplicon {S}equence {V}ariant), revealing microbial succession patterns. {B}acterial communities shifted from {P}seudomonadota-dominated assemblages ({A}pril) to {B}acteroidota-dominated communities later in the year, with recurring {ASV}s (e.g., {P}seudohongiella, {N}eptuniibacter caesariensis, {M}ycobacterium). {F}ungal communities showed marked temporal fluctuations, including recurrent peaks of potentially pathogenic genera ({A}spergillus, {C}andida, {R}amichloridium). {M}icroalgal assemblages were habitat-structured ({C}hlorophyta in seawater, {R}hodophyta in sediments), while protists communities exhibited pronounced late-season shifts, including dinoflagellate enrichment in {O}ctober. {M}easured physicochemical parameters explained only a limited fraction of community variability, suggesting a strong role for biological interactions and unmeasured drivers. {T}hese findings highlight the sensitivity of high-resolution, multikingdom microbial fingerprinting for detecting early-warning bioindicators, outperforming traditional lowfrequency monitoring. {T}his study provides a robust, transferable framework for proactive coral reef biomonitoring and ecosystem management under increasing anthropogenic pressure.},
  keywords = {e{DNA} ; {M}ultimarkers ; {C}oral reefs ; {M}icrobial community dynamics ; {R}eef bioindicators ; {REUNION}},
  booktitle = {},
  journal = {{M}arine {E}nvironmental {R}esearch},
  volume = {219},
  numero = {},
  pages = {108111 [16 p.]},
  ISSN = {0141-1136},
  year = {2026},
  DOI = {10.1016/j.marenvres.2026.108111},
  URL = {https://www.documentation.ird.fr/hor/fdi:010097348},
}