@article{fdi:010097119,
  title = {{I}mproved detection of rice yellow mottle virus with a polyclonal antibody x{MAP} assay : a high-throughput alternative to {ELISA}},
  author = {{V}ernet, {A}udrey and {T}haurignac, {G}. and {P}oulicard, {N}ils and {B}ouillin, {J}. and {G}alzi, {A}gn{\`e}s and {P}inault, {A}deline and {B}amogo, {P}. {K}. {A}. and {P}eeters, {M}artine and {A}youba, {A}hidjo and {L}acombe, {S}{\'e}verine},
  editor = {},
  language = {{ENG}},
  abstract = {{R}ice yellow mottle virus ({RYMV}) is one of the most destructive viral pathogens affecting rice production in sub{S}aharan {A}frica, where its extensive genetic diversity and high evolutionary rate complicate reliable diagnosis. {C}urrent serological methods, notably double-antibody sandwich enzyme-linked immunosorbent assay ({DASELISA}), are widely used for routine detection but may lack sensitivity when viral loads are low or samples are degraded. {I}n this study, we developed and evaluated a high-throughput microsphere immunoassay ({MIA}) based on {L}uminex x{MAP} technology as an alternative serological tool for {RYMV} detection. {A} panel of three monoclonal antibodies (m{AB}s) and one polyclonal antibody (p{AB}), all raised against a {RYMV} isolate from {M}adagascar, was assessed using greenhouse-propagated and field-collected rice samples representing all six known {RYMV} genetic groups. {A}ssay performance was evaluated in terms of sensitivity, specificity, and accuracy using receiver operating characteristic ({ROC}) curve analysis, and results were compared with those obtained by {DAS}-{ELISA}. {W}hile m{AB}s failed to retain their expected serotype specificity in the x{MAP} format, the polyclonal antibody enabled robust and strain-independent detection of {RYMV}. {T}he p{AB}-based x{MAP} assay showed excellent diagnostic performance, with 100% specificity, sensitivity exceeding 97%, and an area under the {ROC} curve of 0.99. {I}mportantly, the p{AB}-based x{MAP} assay demonstrated a 100- to 500-fold increase in sensitivity compared with {DAS}-{ELISA}, allowing reliable detection of {RYMV} in samples with low viral loads or compromised integrity. {N}o cross-reactivity was observed with other co-circulating rice viruses, including maize streak virus and rice stripe necrosis virus. {T}his study demonstrates that a polyclonal antibody-based x{MAP} immunoassay provides a highly sensitive, specific, and scalable alternative to conventional {ELISA} for {RYMV} diagnosis. {T}his approach represents a promising tool for large-scale epidemiological surveys and improved management of rice viral diseases in {A}frica.},
  keywords = {{RYMV} ; x{MAP} {L}uminex immunoassay ; {P}olyclonal antibody ; {D}iagnostic ; {COTE} {D}'{IVOIRE} ; {MALI} ; {BURKINA} {FASO} ; {SIERRA} {LEONE} ; {MADAGASCAR} ; {TANZANIE} ; {AFRIQUE} {SUBSAHARIENNE}},
  booktitle = {},
  journal = {{V}irus {R}esearch},
  volume = {368},
  numero = {},
  pages = {199740 [10 p.]},
  ISSN = {0168-1702},
  year = {2026},
  DOI = {10.1016/j.virusres.2026.199740},
  URL = {https://www.documentation.ird.fr/hor/fdi:010097119},
}