@article{fdi:010096916,
  title = {{D}engue virus harnesses mosquito {S}yntenin to load and secrete viral {RNA} into salivary exosomes},
  author = {{R}achenne, {F}. and {S}chneider, {N}orman and {R}ey-{C}adilhac, {F}. and {S}errato-{P}omar, {I}. and {P}ruvost, {L}auryne and {D}i {P}ietro, {L}. and {D}ainat, {J}acques and {V}ernet, {A}udrey and {C}azevieille, {C}. and {A}ncelin, {A}. and {H}im, {J}. {L}. {K}. and {J}ansen, {S}. and {M}iot, {E}. {F}. and {F}raering, {J}eremy and {S}imon, {L}. and {M}iss{\'e}, {D}oroth{\'e}e and {M}orille, {M}. and {H}ammann, {P}. and {S}eveno, {M}. and {U}rbach, {S}. and {D}allmeier, {K}. and {M}arois, É and {P}ompon, {J}ulien},
  editor = {},
  language = {{ENG}},
  abstract = {{V}iruses exploit extracellular vesicles ({EV}s) to transfer infection-enhancing viral {RNA}s. {H}owever, mechanisms underlying viral {RNA} loading remain elusive. {W}e leveraged our previous discovery that dengue virus secretes transmission-enhancing subgenomic flaviviral {RNA} (sf{RNA}) into mosquito salivary {EV}s to investigate viral {RNA} loading mechanism. {W}e demonstrate that sf{RNA} alone promotes the secretion of sf{RNA}-containing {EV}s marked by the mosquito {EV} biogenesis protein {A}e{S}yntenin, by applying microscopy and viral genetic editing in in vitro and in vivo models. {S}f{RNA} via its stem loop structures interacts intracellularly with mosquito {A}e{S}yntenin and this interaction is selectively maintained within {EV}s as shown by complementary {RNA}-affinity chromatography and {RNA} immunoprecipitation, and {AI}-based prediction. {F}inally, we used systemic and salivary gland-specific protein depletion to establish a functional role for mosquito {A}e{S}yntenin in exosome production and salivary secretion of sf{RNA}. {W}e propose that sf{RNA} binds {A}e{S}yntenin to drive its selective packaging and release into exosomes, elucidating a mechanism for viral {RNA} incorporation into {EV}s.},
  keywords = {viral dissemination ; exosomes ; |orthoflavivirus ; mosquito-borne diseases},
  booktitle = {},
  journal = {{P}roceedings of the {N}ational {A}cademy of {S}ciences of the {U}nited {S}tates of {A}merica},
  volume = {123},
  numero = {12},
  pages = {e2520697123 [12 p.]},
  ISSN = {0027-8424},
  year = {2026},
  DOI = {10.1073/pnas.2520697123},
  URL = {https://www.documentation.ird.fr/hor/fdi:010096916},
}