@article{fdi:010096813,
  title = {{F}irst report of a new bacterial leaf blight of rice caused by {P}antoea ananatis and {P}antoea stewartii in {B}enin [r{\'e}sum{\'e}]},
  author = {{K}ini, {K}. and {A}gnimonhan, {R}. and {A}folabi, {O}. and {M}ilan, {B}. and {S}oglonou, {B}. and {G}bogbo, {V}. and {K}oebnik, {R}alf and {S}ilue, {D}.},
  editor = {},
  language = {{ENG}},
  abstract = {{F}rom 2011 to 2015, surveys were conducted in rice fields of {B}enin to assess the importance of bacterial leaf blight ({BLB}) of rice caused by {X}anthomonas oryzae pv. oryzae. {BLB}-like diseased leaf samples were collected showing

yellowing symptoms or one to two orange to brown stripes on one or both halves of the leaf blade. {O}lder symptoms enlarged to the entire leaf and showed brown stripes below the leaf tip and along the leaf margins. {S}everely

affected leaves became grayish-brown. {T}o diagnose the disease, symptomatic leaf pieces were surface-sterilized and macerated in sterile water. {U}pon plating on semiselective peptone-sucrose-agar ({PSA}) medium ({P}oulin et al. 2014),

straw-colored to yellow colonies were obtained after incubation at 28°{C} for 1 to 2 days. {I}n parallel, the leaf sap was subjected to a diagnostic multiplex {PCR} assay for {X}. oryzae pathovars ({L}ang et al. 2010). {O}ver 3,000 samples

tested were negative. {B}ecause leaf blight can also be caused by species of {P}antoea ({L}ee et al. 2010; {M}ondal et al. 2011), the isolates were tested with {PCR} primers that amplify a gyr{B} fragment from {P}antoea spp. ({B}rady et al. 2008),

often resulting in {PCR} amplicons of the expected size. {H}owever, since these primers were not specific to the genus {P}antoea but also amplified loci from other {E}nterobacteriaceae, we used genomic information from 26 {P}antoea

strains to develop species-specific primers targeting the gyr{B} gene of {P}. ananatis ({PANAN}_gyr{B}-{F}, 5'-{TGACGATGCCCGTGAAGG}; {PANAN}_gyr{B}-{R}, 5'-{TAATCAACGTGGCRACTTCC}) and {P}. stewartii ({PANST}_gyr{B}-{F}1, 5'-{AGGGATACAGCAAGAAGGC}; {PANST}_gyr{B}-{R}1, 5'-{TAGCCACTTCCTGAGACG}). {S}ingle colonies were isolated from {PCR}-positive samples, including strains {ARC}22 and {ARC}570. gyr{B} fragments of both isolates were amplified and sequenced using the above primers. {T}he {BLASTN} searches of a trimmed 426 and 508 bp {DNA} fragment revealed that the nucleotide sequences were 99% identical to the gyr{B} gene from {P}. ananatis strain 17671 ({G}en{B}ank accession no. {KF}554589) and {P}. stewartii strain 626 ({KF}554590), respectively. {T}he partial sequences of the gyr{B} gene were deposited at {G}en{B}ank under {KT}729518 for {ARC}22 and {KT}729519 for {ARC}570. {P}athogenicity assays were conducted on 35-day-old rice plants. {T}o this purpose, bacteria were grown overnight on {PSA} and inoculum adjusted to 108 cells/ml. {I}noculation was done by either clipping half of the leaves or by infiltrating the leaves at about 5 to 15 cm below the leaf tips. {O}ne to two leaves per plant, three plants per accession, and seven cultivars were inoculated. {C}ontrol plants were inoculated with sterile water. {F}ifteen to twenty-one days after incubation, inoculated leaves showed typical {BLB}-like lesions whereas control plants remained symptomless. {T}he reisolated bacteria from diseased leaves yielded colonies identical to those described above and confirmed as {P}. ananatis and {P}. stewartii by {PCR} and sequence analysis of the gyr{B} gene portion, thus fulfilling {K}och's postulates. {T}o our knowledge, this is the first report of a new bacterial disease caused by species of {P}antoea in {B}enin. {S}ymptoms corresponding to the disease were found at all 14 localities in {B}enin where field prospections were performed and prevalence of the disease varied between 30 and 100%.},
  keywords = {{BENIN}},
  booktitle = {},
  journal = {{P}lant {D}isease},
  volume = {101},
  numero = {1},
  pages = {242},
  ISSN = {0191-2917},
  year = {2017},
  DOI = {10.1094/pdis-06-16-0940-pdn},
  URL = {https://www.documentation.ird.fr/hor/fdi:010096813},
}