@article{fdi:010096630,
  title = {{L}ong-term maintenance of multidrug-resistant {E}scherichia coli carried by vampire bats and shared with livestock in {P}eru},
  author = {{B}enavides, {J}ulio {A}. and {G}odreuil, {S}. and {O}pazo-{C}apurro, {A}. and {M}ahamat, {O}.{O}. and {F}alcon, {N}. and {O}ravcova, {K}. and {S}treicker, {D}.{G}. and {S}hiva, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{E}xtended-spectrum beta-lactamase-producing {E}scherichia coli ({ESBL}-{E}. coli) have been reported in wildlife worldwide. {W}hether wildlife is a transient host of {ESBL}-{E}. coli or comprises an independently maintained reservoir is unknown. {W}e investigated this question by longitudinally monitoring {ESBL}-{E}. coli in common vampire bats and nearby livestock in {P}eru. {A}mong 388 bats from five vampire bat colonies collected over three years, {ESBL}-{E}. coli were detected at a low prevalence (10% in 2015, 4% in 2017 and 2018) compared to a high prevalence (48%) from 134 livestock sampled in 2017. {A}ll {ESBL}-{E}. coli were multidrug-resistant, and whole genome sequencing of 33 randomly selected {ESBL}-{E}. coli isolates (18 recovered from bats) detected 46 genes conferring resistance to antibiotics including third-generation cephalosporins (e.g., bla{CTX}-{M}-55, bla{CTX}-{M}-15, bla{CTX}-{M}-65, bla{CTX}-{M}-3, bla{CTX}-{M}-14), aminoglycosides, fluoroquinolones, and colistin (mcr-1). 




{T}he mcr-1 gene is reported for the first time on a wild bat in {L}atin {A}merica. {ESBL}-{E}. coli also carried 31 plasmid replicon types and 16 virulence genes. {T}wenty-three {E}. coli sequence types ({ST}s) were detected, including {ST}s involved in clinical infections worldwide (e.g., {ST} 167, {ST} 117, {ST} 10, {ST} 156 and {ST} 648). {ESBL}-{E}. coli with identical cg{MLST} ({ST} 167) were detected in the same bat roost in 2015 and 2017, and several {ESBL}-{E}. coli from different bat roosts clustered together in the cg{MLST} reconstruction, suggesting long-term maintenance of {ESBL}-{E}. coli within bats. {M}ost antibiotic resistance and virulence genes were detected in {E}. coli from both host populations, while {ESBL}-{E}. coli {ST} 744 was found in a bat and a pig from the same locality, suggesting possible cross-species exchanges of genetic material and/or bacteria between bats and livestock. {T}his study suggests that wild mammals can maintain multidrug-resistant bacteria and share them with livestock.},
  keywords = {{PEROU}},
  booktitle = {},
  journal = {{S}cience of {T}he {T}otal {E}nvironment},
  volume = {810},
  numero = {},
  pages = {152045 [8 ]},
  ISSN = {0048-9697},
  year = {2022},
  DOI = {10.1016/j.scitotenv.2021.152045},
  URL = {https://www.documentation.ird.fr/hor/fdi:010096630},
}