@article{fdi:010096412, title = {{B}acterial genome reconstruction and community profiling in {N}eotropical {D}rosophila}, author = {{U}lloa, {M}. {A}. and {S}errano, {A}. {V}. and {C}amelo, {L}. {C}. and {G}uyot, {R}omain and {V}ela, {D}. and {M}uñoz, {A}. {R}.}, editor = {}, language = {{ENG}}, abstract = {{D}rosophila species serve as key models for microbiota research due to their relatively simple microbial communities. {H}owever, microbial diversity and dynamics in {N}eotropical {A}ndean {D}rosophila remain underexplored. {H}ere we applied shotgun metagenomics to characterize the microbiota of 24 {N}eotropical {D}rosophila species from {E}cuador, reconstructing 64 high-quality bacterial genomes predominantly from {A}cetobacteraceae and {E}nterobacterales. {M}icrobial communities were consistently dominated by yeasts, lactic acid bacteria, acetic acid bacteria, and {W}olbachia. {C}omparative analyses revealed no strong correlation between host phylogeny and microbial community composition, suggesting environmental factors and microbial interactions shape these communities. {N}otably, shifts in relative abundances indicate dynamic ecological succession and metabolic cooperation among microbes. {T}hese findings expand genomic resources for {D}rosophila-associated bacteria and highlight the complex ecological processes influencing host-microbiota relationships in natural populations.}, keywords = {{N}eotropical {D}rosophila spp ; {M}etagenome-assembled genomes ({MAG}s) ; {M}icrobiota ; {G}ut bacteria ; {P}hylogenetic analysis ; {EQUATEUR}}, booktitle = {}, journal = {{S}cientific {R}eports - {N}ature}, volume = {16}, numero = {1}, pages = {6601 [15 p.]}, ISSN = {2045-2322}, year = {2026}, DOI = {10.1038/s41598-026-36282-y}, URL = {https://www.documentation.ird.fr/hor/fdi:010096412}, }