Villarroel P. M. S. auteur aut IRD Piorkowski G. auteur aut IRD Laojun S. auteur aut IRD Liégeois Florian auteur aut IRD Gumpangset N. auteur aut IRD Missé Dorothée auteur aut IRD Chaiphongpachara T. auteur aut IRD Wichit S. auteur aut IRD text journalArticle eng text/pdf reformatted digital access Ticks are obligate blood-feeding ectoparasites that harbor a wide diversity of microorganisms. Rhipicephalus sanguineus, the brown dog tick, is globally distributed and poses significant veterinary and public health concerns due to its close association with companion animals and its occasional infestation of humans. However, the virome of this species in Thailand remains poorly characterized. In this study, we employed DNA Nanoball sequencing to investigate the virome of 80 R. sanguineus ticks, grouped into five pools, collected from dogs in Chachoengsao Province, Thailand, in 2023. Three viruses were identified: Brown dog tick phlebovirus 2 (BDTPV2), Changping tick virus 2 (CpTV-2), and Bole tick virus 4 (BLTV4), all detected in male ticks. These results highlight the need for further investigation into the ecological roles and biological significance of these viruses. Overall, our findings provide an updated perspective on the R. sanguineus virome in Thailand and underscore the importance of continued surveillance of tick-associated viruses within the One Health framework. specialized Bole tick virus 4 Brown dog tick phlebovirus 2 Changping tick virus 2 metagenomics Rhipicephalus sanguineus Thailand viruses THAILANDE 052 16 1736178 [8 p.] 2026 https://www.documentation.ird.fr/hor/fdi:010096075 10.3389/fmicb.2025.1736178 [F B010096075] https://www.documentation.ird.fr/hor/fdi:010096075 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2026-03/010096075.pdf IRD - Base Horizon / Pleins textes 2026-03-11 2026-03-11 fdi:010096075 fre