@article{fdi:010095506,
  title = {{D}evelopment of new microsatellite markers for {C}ola acuminata ({M}alvaceae), a socio-economically important fruit tree species in {C}entral {A}frica},
  author = {{D}upiol, {P}. and {C}hakocha, {A}. and {T}ientcheu, {M}. {L}. {A}. and {C}harahabil, {M}. {M}. and {M}ariac, {C}{\'e}dric and {B}arnaud, {A}deline and {D}uminil, {J}{\'e}r{\^o}me},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground and aims-{W}e developed a new set of nuclear microsatellite markers for {C}ola acuminata ({M}alvaceae), an important {A}frican food tree species commonly known as the kola nut. {P}robably originating from the tropical rainforests of the {C}ongo {B}asin, {C}. acuminata is widely cultivated in the humid savannahs of the region where its nuts are sold throughout {C}entral and {W}est {A}frica for their stimulant properties. {N}uclear microsatellite markers ({SSR}s) are well suited for assessing the genetic diversity and population structure of plant species due to their high variability. {M}aterial and methods-{L}eaf samples were collected from 84 {C}. acuminata cultivated individuals across three sites in {C}ameroon, two in the savannah zone, one in the forest zone. {SSR} markers were developed by sequencing genomic {DNA} from two individuals using an {I}llumina {H}i{S}eq platform. {G}enetic diversity was assessed based on 14 {SSR} markers genotyped in 84 individuals, and marker transferability to the closely related species {C}ola nitida was tested. {K}ey results-{F}orty-eight new microsatellite loci were developed, of which 14 were polymorphic in {C}. acuminata. {T}he results demonstrated a high level of genetic diversity with the presence of two to 33 alleles per locus (with an average of 14.14) across the three sites. {T}he transferability of these markers was confirmed with 13 out of the 14 {SSR}s successfully amplifying in the closely related species, {C}ola nitida. {C}onclusion-{T}hese newly developed {SSR}s will be useful for assessing genetic diversity, genetic differentiation, and gene flow patterns of {C}. acuminata in the tropical forests of {C}entral {A}frica. {P}reliminary results suggest genetic similarity between the two savannah sites. {H}owever, these two sites were significantly differentiated from the site in the forest zone. {T}his suggests that the propagation material introduced in the savannah zone did not originate from the forest in southern {C}ameroon.},
  keywords = {conservation ; domestication history ; food tree species ; non-timber ; forest products ; tree crop ; {CAMEROUN} ; {SENEGAL} ; {AFRIQUE} {CENTRALE} ; {AFRIQUE} {DE} {L}'{OUEST}},
  booktitle = {},
  journal = {{P}lant {E}cology and {E}volution},
  volume = {158},
  numero = {3},
  pages = {358--366},
  ISSN = {2032-3913},
  year = {2025},
  DOI = {10.5091/plecevo.147801},
  URL = {https://www.documentation.ird.fr/hor/fdi:010095506},
}