@article{fdi:010095465,
  title = {{C}ultivating microbial communities from the serpentinite-hosted {P}rony {B}ay hydrothermal field on different carbon sources in hydrogen-fed bioreactors},
  author = {{P}opall, {R}. {M}. and {R}oland, {A}. and {D}avidson, {S}ylvain and {C}ombet-{B}lanc, {Y}annick and {P}rice, {R}. {E}. and {Q}u{\'e}m{\'e}neur, {M}arianne and {P}ostec, {A}. and {E}rauso, {G}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {T}he primary source of carbon is one of the most fundamental questions regarding the development of microbial communities in serpentinite-hosted systems. {T}he hydration of ultramafic rock to serpentinites releases large amounts of hydrogen and creates hyperalkaline conditions that deplete the environment of dissolved inorganic carbon. {M}etagenomic studies suggest that serpentinite-hosted microbial communities depend on the local redissolution of bicarbonate and on small organic molecules produced by abiotic reactions associated with serpentinization. {M}ethods {T}o verify these bioinformatic predictions, microbial consortia collected from the {P}rony {B}ay hydrothermal field were enriched under anoxic conditions in hydrogen-fed bioreactors using bicarbonate, formate, acetate, or glycine as the sole carbon source. {C}onclusions {W}ith the exception of glycine, the chosen carbon substrates allowed the growth of microbial consortia characterized by significant enrichment of individual taxa. {S}urprisingly, these taxa were dominated by microbial genera characterized as aerobic rather than anaerobic as expected. {O}ur results indicate the presence of both autotrophic and heterotrophic taxa that may function as foundation species in serpentinite-hosted shallow subsurface ecosystems. {W}e propose that an intricate feedback loop between these autotrophic and heterotrophic foundation species facilitates ecosystem establishment. {B}icarbonate-fixing {M}eiothermus and {H}ydrogenophaga, as well as formate-fixing {M}eiothermus, {T}hioalkalimicrobium, and possibly a novel genotype of {R}oseibaca might produce organic compounds for heterotrophs at the first trophic level. {I}n addition, the base of the trophic network may include heterotrophic {R}oseibaca, {A}cetoanaerobium, and {M}eiothermus species producing {CO}2 from acetate for a more diverse community of autotrophs. {T}he cultivated archaeal community is expected to recycle {CH}4 and {CO}2 between {M}ethanomicrobiales and {M}ethanosarcinales with putative {W}oesearchaeales symbionts.},
  keywords = {{S}erpentinization ; {S}ubmarine alkaline vent ; {H}ydrothermal system ; {A}lkaliphile ; {H}ydrogenotroph ; {P}rimary production ; {C}arbon fixation ; {O}rigin of life ; {NOUVELLE} {CALEDONIE} ; {PACIFIQUE} ; {PRONY} {BAIE}},
  booktitle = {},
  journal = {{E}nvironmental {M}icrobiome},
  volume = {20},
  numero = {1},
  pages = {135 [14 ]},
  year = {2025},
  DOI = {10.1186/s40793-025-00797-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010095465},
}