Dahms C. auteur aut IRD Vigliola Laurent auteur aut IRD Cheung L. H. T. auteur aut IRD Hui J. H. L. auteur aut IRD Momigliano P. auteur aut IRD text journalArticle eng text/pdf reformatted digital access To date less than 5% of shark species have nuclear reference genomes, despite next-generation sequencing advances. Particularly for threatened shark species, there is a lack of reliable genomes which are crucial in facilitating research and conservation applications. We assembled the first nuclear reference genome of the endangered grey reef shark (Carcharhinus amblyrhynchos) using long-read PacBio HiFi and Omni-C sequencing to reach chromosome-level contiguity (36 pseudochromosomes; 2.9 Gbp) and high completeness (94% complete BUSCOs). BRAKER3 annotated 16,505 protein-coding genes after masking repetitive elements which accounted for 59% of the genome. We identified potential X and Y sex chromosomes on pseudochromosomes 36 and 57, respectively. The quality and completeness of the draft genome of C. amblyrhynchos will enable researchers to investigate genetic variations and adaptations specific to this species as well as across other Carcharhinus spp., opening new venues for comparative genomics and advancing conservation genetic applications. specialized reference genome HiFi Carcharhinidae shark conservation sex chromosome NOUVELLE CALEDONIE MALDIVES AUSTRALIE OCEAN INDIEN 034 020 082 17 10 evaf176 [9 p.] 2025 1759-6653 https://www.documentation.ird.fr/hor/fdi:010095345 10.1093/gbe/evaf176 1759-6653 [F B010095345] https://www.documentation.ird.fr/hor/fdi:010095345 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2025-11/010095345.pdf IRD - Base Horizon / Pleins textes 2025-11-18 2025-11-18 fdi:010095345 fre