@article{fdi:010095345, title = {{C}hromosome-level assembly and annotation of the grey reef shark ({C}archarhinus amblyrhynchos) genome}, author = {{D}ahms, {C}. and {V}igliola, {L}aurent and {C}heung, {L}. {H}. {T}. and {H}ui, {J}. {H}. {L}. and {M}omigliano, {P}.}, editor = {}, language = {{ENG}}, abstract = {{T}o date less than 5% of shark species have nuclear reference genomes, despite next-generation sequencing advances. {P}articularly for threatened shark species, there is a lack of reliable genomes which are crucial in facilitating research and conservation applications. {W}e assembled the first nuclear reference genome of the endangered grey reef shark ({C}archarhinus amblyrhynchos) using long-read {P}ac{B}io {H}i{F}i and {O}mni-{C} sequencing to reach chromosome-level contiguity (36 pseudochromosomes; 2.9 {G}bp) and high completeness (94% complete {BUSCO}s). {BRAKER}3 annotated 16,505 protein-coding genes after masking repetitive elements which accounted for 59% of the genome. {W}e identified potential {X} and {Y} sex chromosomes on pseudochromosomes 36 and 57, respectively. {T}he quality and completeness of the draft genome of {C}. amblyrhynchos will enable researchers to investigate genetic variations and adaptations specific to this species as well as across other {C}archarhinus spp., opening new venues for comparative genomics and advancing conservation genetic applications.}, keywords = {reference genome ; {H}i{F}i ; {C}archarhinidae ; shark conservation ; sex chromosome ; {NOUVELLE} {CALEDONIE} ; {MALDIVES} ; {AUSTRALIE} ; {OCEAN} {INDIEN}}, booktitle = {}, journal = {{G}enome {B}iology and {E}volution}, volume = {17}, numero = {10}, pages = {evaf176 [9 p.]}, ISSN = {1759-6653}, year = {2025}, DOI = {10.1093/gbe/evaf176}, URL = {https://www.documentation.ird.fr/hor/fdi:010095345}, }