@article{fdi:010095291,
  title = {{A} {SHERLOCK} toolbox for eco-epidemiological surveillance of {A}frican trypanosomes in domestic pigs from {W}estern {A}frica},
  author = {{E}loiflin, {R}. and {P}erez-{A}ntón, {E}. and {C}amara, {A}. and {D}ujeancourt-{H}enry, {A}. and {B}oiro, {S}. and {D}jetchi, {M}. {N}. and {T}raore, {M}. {B}. and {K}offi, {M}. and {K}aba, {D}. and {L}e {P}ennec, {Y}. and {D}oukoure, {B}. and {C}amara, {A}. {D}. and {K}agbadouno, {M}. and {C}ampagne, {P}ascal and {C}amara, {M}. and {J}amonneau, {V}incent and {T}hevenon, {S}. and {B}art, {J}ean-{M}athieu and {G}lover, {L}. and {R}otureau, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}nimal {A}frican trypanosomosis ({AAT}), caused by protist parasites of the genus {T}rypanosoma, puts upward of a million head of livestock at risk across 37 countries in {A}frica. {T}he economic impact of {AAT} and the presence of human-infectious trypanosomes in animals place a clear importance on improving diagnostics for animal trypanosomes to map the distribution of the veterinary parasites and identify reservoirs of human-infectious trypanosomes. {W}e have adapted the {CRISPR}-based detection toolkit {SHERLOCK} ({S}pecific {H}igh-sensitivity {E}nzymatic {R}eporter un{LOCK}ing) for trypanosomatid parasites responsible for {AAT} ({SHERLOCK}4{AAT}) including {P}an-trypanosomatid, {T}rypanozoon, {T}. vivax, {T}. congolense, {T}. theileri, {T}. simiae, and {T}. suis assays. {T}o test the applicability of this technique in the field, we analysed dried blood spots collected from 200 farm and 224 free-ranging pigs in endemic and historical human {A}frican trypanosomiasis foci in {G}uinea and {C}{\^o}te d'{I}voire, respectively. {T}he results revealed that {SHERLOCK}4{AAT} can detect and discriminate between trypanosome species involved in multiple infections with a high sensitivity. 62.7% [58.1, 67.3] of pigs were found infected with at least one trypanosome species. {T}. brucei gambiense, a human-infectious trypanosome, was found in one animal at both sites, highlighting the risk that these animals may act as persistent reservoirs. {T}hese data suggest that, due to their proximity to humans and their attractiveness to tsetse flies, pigs could act as sentinels to monitor {T}. b. gambiense circulation using the {SHERLOCK}4{AAT} toolbox.},
  keywords = {{A}frican trypanosomes ; detection ; {SHERLOCK} ; surveillance ; pigs ; {AFRIQUE} {SUBSAHARIENNE}},
  booktitle = {},
  journal = {e{L}ife},
  volume = {14},
  numero = {},
  pages = {{RP}106823 [22 ]},
  ISSN = {2050-084{X}},
  year = {2025},
  DOI = {10.7554/e{L}ife.106823},
  URL = {https://www.documentation.ird.fr/hor/fdi:010095291},
}