@article{fdi:010094937,
  title = {{V}ector competence of {A}edes albopictus for {T}onate virus highlights transmission risks in temperate and tropical regions},
  author = {{M}oltini-{C}onclois, {I}. and {K}hanom, {W}. and {M}iot, {E}. {F}. and {P}intong, {A}. and {N}jifon, {H}. {L}. {M}. and {G}inebre, {C}. and {C}houmet, {V}. and {C}outard, {B}. and {M}ariac, {C}{\'e}dric and {R}oques, {P}. and {C}olmant, {A}gathe and {P}iorkowski, {G}. and {P}ompon, {J}ulien and {M}iss{\'e}, {D}oroth{\'e}e},
  editor = {},
  language = {{ENG}},
  abstract = {{R}ecent evidence of vertical transmission of {T}onate virus ({TONV}) during early pregnancy and its association with fetal neurological anomalies highlights its potential public health threat. {TONV} is an understudied alphavirus endemic to {F}rench {G}uiana. {T}he growing presence of {A}edes ({A}e.) albopictus in {E}urope raises concerns about its ability to transmit emerging arboviruses, including {TONV}. {W}e assessed the vector competence of {A}e. albopictus populations from mainland {F}rance and {L}a {R}{\'e}union {I}sland via oral infections using different {TONV} doses. {B}oth populations supported efficient viral replication, with infectious viral particles appearing in saliva by day 5 post-infection. {I}nfection rate ({IR}), stepwise dissemination rate (s{DR}), and transmission efficiency ({TE}) increased with rising viral concentrations. {A}t a viral concentration of 106 {PFU}/m{L} {IR} reached 80%, and {TE} at day 5 post-infection was 27% for the mainland strain and 37% for the {L}a {R}{\'e}nion strain. {N}otably, {TE} declined over time in the mainland strain, while increasing progressively in the {L}a {R}{\'e}union population. {C}omparative infections with {C}hikungunya virus revealed that {TONV} was transmitted at similar or greater rates, confirming {A}e. albopictus as a competent vector. {S}equencing of mosquito organs revealed intra-host {TONV} genetic variability. {A} recurrent polymorphism at position 11,357 in the 3 ' {UTR} was detected in body tissues but not in heads of {L}a {R}{\'e}union mosquitoes, suggesting tissue-specific selection or bottlenecks. {O}ur results demonstrate that {A}e. albopictus from both tropical and temperate areas can efficiently transmit {TONV} and emphasize the importance of genomic surveillance to anticipate risk of its emergence in areas where this vector is established.},
  keywords = {{A}rbovirus ; {T}onate virus ; {C}hikungunya virus ; {A}edes albopictus ; vector competence ; {REUNION}},
  booktitle = {},
  journal = {{E}merging {M}icrobes and {I}nfections},
  volume = {14},
  numero = {1},
  pages = {2547733 [12 p.]},
  year = {2025},
  DOI = {10.1080/22221751.2025.2547733},
  URL = {https://www.documentation.ird.fr/hor/fdi:010094937},
}