@article{fdi:010094925,
  title = {{C}omparison of the composition and antiplasmodial activity of {A}rtemisia annua teas using an untargeted metabolomic approach},
  author = {{A}ubouy, {A}gn{\`e}s and {A}moussa, {A}. {M}. {O}. and {L}agnika, {L}. and {F}abre, {N}. and {V}itrai, {A}drien and {C}laparols, {C}. and {H}ouz{\'e}, {S}. and {S}arrasin, {V}. and {C}hapeland-{L}eclerc, {F}. and {R}uprich-{R}obert, {G}. and {B}ourgeade-{D}elmas, {S}andra and {Y}edomonhan, {H}. and {D}umondin, {C}harlotte and {J}ullian, {V}al{\'e}rie},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he use of teas made from locally cultivated {A}rtemisia annua to fight malaria in remote areas where access to care is difficult is a matter of debate. {T}his study aimed at document differences in the composition of {A}. annua teas cultivated in {B}enin to be sold as antimalarial teas, and in {F}rance, and their impact on antiplasmodial activity. {A}. annua teas were prepared with plants from one location in south {F}rance and from ten different plantations in {B}enin. {A}rtemisinin was quantified in herbal teas with a liquid chromatography system coupled to mass spectrometry and multiple reaction monitoring detection methods. {T}he herbal teas were tested against chloroquine-sensitive 3{D}7 strain of {P}lasmodium falciparum using isotopic microtest to determine {IC}50 values and calculate the concentration of artemisinin corresponding to the {IC}50 of the teas [{ART}(tea)_{IC}50]. {C}hemical profiles were determined by liquid chromatography coupled to high resolution mass spectrometry and a metabolomic analysis was performed to annotate compounds statistically linked to the antiplasmodial properties of the teas. {A}rtemisinin content varied between 0.3 mg/{L} for tea with plants from {F}rance to 15.7 mg/{L} for teas made with plants from {B}enin with differences between locations. {A}rtemisinin content was decreasing after a one-year storage of the plant for 3 localities in {B}enin with loss of 33%, 48% and 24% ({P} < 0.05). {A}rtemisinin concentrations and antiplasmodial activity of teas were positively correlated although the comparison of {ART}(tea)_{IC}50s to {IC}50 of pure artemisinin suggested that other compounds present in the tea were involved in the activity, either enhancing or limiting it. {U}nknown alkaloids in {A}. annua teas correlated to antiplasmodial activity were also detected. {T}hese findings suggest that {A}. annua teas deserve further studies to identify other metabolites of interest and determine their role in antiplasmodial activity in relation to other molecules, particularly artemisinin.},
  keywords = {{BENIN} ; {FRANCE}},
  booktitle = {},
  journal = {{PL}o{S} {O}ne},
  volume = {20},
  numero = {8},
  pages = {e0330682 [23 ]},
  year = {2025},
  DOI = {10.1371/journal.pone.0330682},
  URL = {https://www.documentation.ird.fr/hor/fdi:010094925},
}