@article{fdi:010094833,
  title = {{G}enetic polymorphism of msp2 in {P}lasmodium falciparum isolates among asymptomatic malaria infections from two ecological settings in {C}ameroon},
  author = {{B}ouopda-{T}uedom, {A}. {G}. and {A}bate, {L}uc and {F}eufack-{D}onfack, {B}. {L}. and {N}gou, {C}. {M}. and {B}ayibeki, {A}. {N}. and {M}oukoko, {C}. {E}. {E}. and {A}yong, {L}. {S}. and {B}erry, {A}. and {L}efevre, {T}. and {M}orlais, {I}sabelle and {N}sango, {S}. {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground{T}he high genetic diversity of {P}lasmodium falciparum parasites is one of the challenges for malaria control and elimination in endemic areas. {A} better knowledge of parasite genotypes circulating in different disease endemic areas could help to optimize local malaria interventions. {T}his study aimed at determining {P}. falciparum genetic diversity from isolates collected in forest ({M}fou) and humid savanna ({T}ibati) eco-epidemiological settings in {C}ameroon.{M}ethods{D}ried blood spots collected from asymptomatic individuals in 2018 and 2019 were used to determine the {P}lasmodium infection status and distinguish the {P}lasmodium spp. by real-time {PCR}. {A}llelic polymorphism of the msp2 gene was assessed in the {P}. falciparum positive samples by nested {PCR} followed by capillary electrophoresis for revelation of the fragment allelic size. {M}ultiplicity of infection ({MOI}) was defined as the number of coinfecting genotypes within an infection. {G}eneral linear mixed models were fitted to evaluate the impact of study site, participant age, gender and bed net ownership on genetic diversity.{R}esults{M}alaria prevalence among the asymptomatic individuals reached 59.2% (876/1480) in {M}fou and 63.4% (808/1274) in {T}ibati. {A} total of 36 and 42 different msp2 alleles were detected in {M}fou and {T}ibati, respectively. {N}o genetic differentiation was observed between the two study sites. {T}he msp2 {IC}/3{D}7 family was the most polymorphic and the most prevalent in both areas. {O}verall, more than 60% of the isolates had multiclonal infections. {T}he frequency of multiclonal infections and {MOI} was higher in {M}fou (68.9%, {MOI} = 2.08) than in {T}ibati (57.29%, {MOI} = 1.80). {I}n {M}fou, a negative correlation was found between {MOI} and age. {S}imilarly, a gender effect was observed in {M}fou, with males having higher {MOI} than females.{C}onclusion{T}his study reported high malaria prevalence and a high allelic diversity in the msp2 gene among asymptomatic carriers from two epidemiological settings of {C}ameroon. {D}espite results reflects high transmission intensity in both areas, analysis indicated distinct epidemiological patterns in {M}fou and {T}ibati. {T}hese findings will provide valuable baseline information to monitor the impact of malaria control measures implemented in these areas.},
  keywords = {{G}enetic diversity ; {P}lasmodium falciparum ; {M}erozoite ; surface protein 2 ; {M}ulticlonal infections ; {H}eterozygosity ; {CAMEROUN}},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {24},
  numero = {1},
  pages = {253 [14 p.]},
  year = {2025},
  DOI = {10.1186/s12936-025-05414-6},
  URL = {https://www.documentation.ird.fr/hor/fdi:010094833},
}