@article{fdi:010094640,
  title = {{A}dapted molecular methods to unravel the recalcitrant mycorrhizal associations of {A}ucoumea klaineana {P}ierre},
  author = {{G}uidosse, {Q}. and {R}oy, {M}{\'e}lanie and {L}assois, {L}. and {D}oucet, {J}.{L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{U}nderstanding the role of root microbiota is crucial in sustainable forest management but remains challenging, especially for tropical trees. {W}e developed an efficient and low-toxicity method to extract and amplify the fungal {DNA} associated with {A}ucoumea klaineana {P}ierre fine roots. {T}o improve {DNA} quality, we optimized a commercial extraction kit by incorporating activated charcoal and modifying incubation periods. {T}his enhanced protocol, combined with bovine serum albumin during {PCR}, effectively mitigated inhibitors present in {A}. klaineana tree root samples. {T}his approach opens new perspectives for studying the microbiota of tropical trees.},
  keywords = {{ZONE} {TROPICALE} ; {REPUBLIQUE} {DEMOCRATIQUE} {DU} {CONGO}},
  booktitle = {},
  journal = {{J}ournal of {M}icrobiological {M}ethods},
  volume = {224},
  numero = {},
  pages = {107000 [4 ]},
  ISSN = {0167-7012},
  year = {2024},
  DOI = {10.1016/j.mimet.2024.107000},
  URL = {https://www.documentation.ird.fr/hor/fdi:010094640},
}