@article{fdi:010094408,
  title = {{B}uilding a {DNA} reference for {M}adagascar's marine fishes : expanding the {COI} barcode library and establishing the first 12s dataset for e{DNA} monitoring},
  author = {{V}olanandiana, {J}. {J}. {A}. and {P}onton, {D}ominique and {R}uiz, {E}. and {F}iadanamiarinjato, {A}. {E}. {M}. and {R}ieuvilleneuve, {F}. and {R}aberinary, {D}. and {C}ollet, {A}. and {B}ehivoke, {F}. and {J}aonalison, {H}. and {R}anaivomanana, {S}. and {L}{\'e}opold, {M}arc and {R}andriatsara, {R}. {M}. and {M}bony, {J}. and {M}ahafina, {J}. and {H}artmann, {A}. and {T}odinanahary, {G}. and {D}urand, {J}ean-{D}ominique},
  editor = {},
  language = {{ENG}},
  abstract = {{M}adagascar harbors a rich marine biodiversity, yet detailed knowledge of its fish species remains limited. {O}f the 1689 species listed in 2018, only 22% had accessible cytochrome oxidase {I} ({COI}) sequences in public databases. {I}n response to growing pressure on fishery resources, this study aims to strengthen biodiversity monitoring tools. {I}ts objectives were to enrich the {COI} database for {M}alagasy marine fishes, create the first 12{S} reference library, and evaluate the taxonomic resolution of different 12{S} metabarcodes for e{DNA} analysis, namely {M}i{F}ish, {T}eleo1, {A}c{MDB}, {A}c12{S}, and 12{SF}1/{R}1. {A}n integrated approach combining morphological, molecular, and phylogenetic analyses was applied for specimen identification of fish captured using various types of fishing gear in {T}oliara and {R}anobe {B}ays from 2018 to 2023. {T}he {M}alagasy {COI} database now includes 2146 sequences grouped into 502 {B}arcode {I}ndex {N}umbers ({BIN}s) from 82 families, with 14 {BIN}s newly added to {BOLD} ({T}he {B}arcode of {L}ife {D}ata {S}ystems), and 133 cryptic species. {T}he 12{S} library comprises 524 sequences representing 446 species from 78 families. {T}ogether, the genetic datasets cover 514 species from 84 families, with the most diverse being {L}abridae, {A}pogonidae, {G}obiidae, {P}omacentridae, and {C}arangidae. {H}owever, the two markers show variable taxonomic resolution: 67 species belonging to 35 families were represented solely in the {COI} dataset, while 10 species from nine families were identified exclusively in the 12{S} dataset. {F}or 319 species with complete 12{S} gene sequences associated with {COI} {BIN}s ({B}arcode {I}ndex {N}umbers), 12{S} primer sets were used to evaluate the taxonomic resolution of five 12{S} metabarcodes. {T}he {M}i{F}ish marker proved to be the most effective, with an optimal similarity threshold of 98.5%. {T}his study represents a major step forward in documenting and monitoring {M}adagascar's marine biodiversity and provides a valuable genetic reference for future environmental {DNA} (e{DNA}) applications.},
  keywords = {fish diversity ; {DNA} barcoding ; reference library ; e{DNA} marker taxonomic ; resolution ; {MADAGASCAR}},
  booktitle = {},
  journal = {{D}iversity},
  volume = {17},
  numero = {7},
  pages = {495 [30 ]},
  year = {2025},
  DOI = {10.3390/d17070495},
  URL = {https://www.documentation.ird.fr/hor/fdi:010094408},
}