@article{fdi:010094392,
  title = {{I}mproving the monitoring of the invasive blue crab ({C}allinectes sapidus) : combining environmental {DNA} and citizen observations},
  author = {{D}elrieu-{T}rottin, {E}. and {B}ianic, {M}. and {M}iaud, {C}. and {G}arcia, {M}. and {A}rnal, {V}. and {H}ocd{\'e}, {R}{\'e}gis and {C}ochet, {C}hristophe and {B}arr{\'e}, {N}. and {C}ornil, {L}. and {M}anel, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{E}arly detection is a crucial tool for identifying the spread of invasive species. {I}n this study, we validated a probe-based quantitative polymerase chain reaction (q{PCR}) assay for the detection of the invasive {B}lue {C}rab, {C}allinectes sapidus, in the {M}editerranean {S}ea, using 22 initial e{DNA} environmental samples (e{DNA}) collected from three coastal lagoons. {A} subsequent large-scale e{DNA} sampling campaign (61 samples in 31 sites), conducted in collaboration with local stakeholders, was carried out to map the distribution of {C}. sapidus along the {O}ccitanie coastline ({W}estern {M}editerranean, {F}rance). {U}sing e{DNA} probe-based q{PCR}, {C}. sapidus was detected in 32 out of the 61 samples (52%), confirming its presence in 24 out of 31 sites surveyed, including the 13 lagoons where its occurrence had already been reported, as well as two additional lagoons and at sea where no prior records existed. {O}ur results demonstrate the utility of e{DNA} probe-based q{PCR} for effective monitoring of the invasive {B}lue {C}rab. {T}he integration of e{DNA} analysis with citizen science observations enhances the monitoring framework, facilitating early detection and contributing to improved management strategies at the very beginning of species colonization when practical actions could be implemented.},
  keywords = {{COI} mt{DNA} ; crustacean ; e{DNA} ; invasive species ; invertebrates ; {M}editerranean {S}ea ; monitoring ; q{PCR} ; quantitative polymerase chain ; reaction (q{PCR})-based e{DNA} detection ; {FRANCE} ; {MEDITERRANNEE} ; {OCCITANIE}},
  booktitle = {},
  journal = {{A}quatic {C}onservation : {M}arine and {F}reshwater {E}cosystems},
  volume = {35},
  numero = {7},
  pages = {e70179 [9 p.]},
  ISSN = {1052-7613},
  year = {2025},
  DOI = {10.1002/aqc.70179},
  URL = {https://www.documentation.ird.fr/hor/fdi:010094392},
}