%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Wesselmann, K. M.
%A Baronti, Cécile
%A Nougairède, A.
%A Thirion, Laurence
%A de Lamballerie, X.
%A Charrel, R.
%A Pezzi, L.
%T Development and evaluation of a duplex RT-qPCR assay for the detection and identification of Mayaro and chikungunya viruses
%D 2025
%L fdi:010094309
%G ENG
%J Journal of Clinical Microbiology
%@ 0095-1137
%K Alphavirus ; Togaviridae ; arbovirus ; chikungunya virus ; Mayaro virus ; diagnostic ; RT-qPCR
%M ISI:001522116500001
%P [11 ]
%R 10.1128/jcm.00420-25
%U https://www.documentation.ird.fr/hor/fdi:010094309
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2025-08/010094309.pdf
%V [Early access]
%W Horizon (IRD)
%X Mayaro virus (MAYV) is a mosquito-borne alphavirus that is widespread in the Amazon basin, where it co-circulates with the closely related chikungunya virus (CHIKV). Due to the limited surveillance and technical limitations of diagnostic assays (scarcity of commercial assays, serology cross-reactivity), the true burden of MAYV is uncertain. We designed a new RT-qPCR assay targeting the nsp1 gene for MAYV detection, which can be used in monoplex or duplex format. In the duplex format, the new MAYV assay is combined with a CHIKV assay and a second MAYV assay, both previously published. The lower limit of detection with a 95% positivity rate was determined to be <10 RNA copies/mu L in monoplex and duplex formats for both MAYV and CHIKV. Monoplex and duplex assays proved to be linear within the tested range of approximately 10(8) to 10(2) RNA copies/mu L and showed 100% specificity against a wide panel of arboviruses as well as several other pathogens in clinical samples. The testing of CHIKV-positive sera and MAYV-spiked plasma samples confirmed the suitability of the assays in a clinical setting. These assays offer a reliable tool for detection and differentiation of MAYV and CHIKV in endemic settings.
%$ 052 ; 020