@article{fdi:010094309,
  title = {{D}evelopment and evaluation of a duplex {RT}-q{PCR} assay for the detection and identification of {M}ayaro and chikungunya viruses},
  author = {{W}esselmann, {K}. {M}. and {B}aronti, {C}{\'e}cile and {N}ougair{\`e}de, {A}. and {T}hirion, {L}aurence and de {L}amballerie, {X}. and {C}harrel, {R}. and {P}ezzi, {L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{M}ayaro virus ({MAYV}) is a mosquito-borne alphavirus that is widespread in the {A}mazon basin, where it co-circulates with the closely related chikungunya virus ({CHIKV}). {D}ue to the limited surveillance and technical limitations of diagnostic assays (scarcity of commercial assays, serology cross-reactivity), the true burden of {MAYV} is uncertain. {W}e designed a new {RT}-q{PCR} assay targeting the nsp1 gene for {MAYV} detection, which can be used in monoplex or duplex format. {I}n the duplex format, the new {MAYV} assay is combined with a {CHIKV} assay and a second {MAYV} assay, both previously published. {T}he lower limit of detection with a 95% positivity rate was determined to be <10 {RNA} copies/mu {L} in monoplex and duplex formats for both {MAYV} and {CHIKV}. {M}onoplex and duplex assays proved to be linear within the tested range of approximately 10(8) to 10(2) {RNA} copies/mu {L} and showed 100% specificity against a wide panel of arboviruses as well as several other pathogens in clinical samples. {T}he testing of {CHIKV}-positive sera and {MAYV}-spiked plasma samples confirmed the suitability of the assays in a clinical setting. {T}hese assays offer a reliable tool for detection and differentiation of {MAYV} and {CHIKV} in endemic settings.},
  keywords = {{A}lphavirus ; {T}ogaviridae ; arbovirus ; chikungunya virus ; {M}ayaro virus ; diagnostic ; {RT}-q{PCR}},
  booktitle = {},
  journal = {{J}ournal of {C}linical {M}icrobiology},
  volume = {[{E}arly access]},
  numero = {},
  pages = {[11 p.]},
  ISSN = {0095-1137},
  year = {2025},
  DOI = {10.1128/jcm.00420-25},
  URL = {https://www.documentation.ird.fr/hor/fdi:010094309},
}