%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Bui, T. T.
%A Tran, T. T. T.
%A Nguyen, T. S.
%A Le, T. T. H.
%A Nguyen, C. L.
%A Pham, H. N.
%A Banuls, Anne-Laure
%A Le, H. S.
%A Le, H. P. A.
%A Bui, T. T.
%A Bui, T. S.
%A Phan, Q. H.
%A Tran, T. H. T.
%A Nguyen, Q. H.
%T Genomic insights into virulence, biofilm formation, and antimicrobial resistance of multidrug-resistant Helicobacter pylori strains of novel sequence types isolated from Vietnamese patients with gastric diseases
%D 2025
%L fdi:010094163
%G ENG
%J Journal of Global Antimicrobial Resistance
%@ 2213-7165
%K Gastric disease ; Genetic mutations ; Helicobacter pylori ; Multidrug-resistance ; Novel sequence type ; Virulence gene
%K VIET NAM
%M ISI:001504638000004
%P 237-241
%R 10.1016/j.jgar.2025.05.001
%U https://www.documentation.ird.fr/hor/fdi:010094163
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2025-07/010094163.pdf
%V 43
%W Horizon (IRD)
%X Helicobacter pylori ( H. pylori ) is a clinically important pathogen associated with gastric diseases. Here, we characterized the genome of multidrug-resistant H. pylori strains of novel sequence types, which were recovered from Vietnamese patients with gastritis or a stomach ulcer. Phenotypic-antibiotic susceptibility testing was performed against amoxicillin, clarithromycin, metronidazol, tetracycline, and levofloxacin using an E-test. The whole genome sequence of three H. pylori strains was de novo assembled, followed by in silico analysis of multilocus sequence typing (MLST), core-genome based phylogeny, genetic determinants associated with virulence, biofilm formation, and antibiotic-resistance. The genome sequence of H. pylori strains exhibited a high similarity with the average nucleotide identity (ANI) values of 98.5% to 99.2%, carried 5 to 7 pathogenicity islands, and 3 to 6 mobilomes. The MLST profile of strains revealed novel sequence types ST4511, ST4512, and ST4513. Core-genome based phylogeny exhibited that the three H. pylori strains belong to the Asian genotype. These strains possessed 128 to 131 virulence genes, including toxin-encoding genes cagA and vacA. Double amoxicillin-resistant mutations on pbp1 and pbp2, or a mutation on pbp3, triple clarithromycin-resistant mutations on 23S rRNA gene and a levofloxacin-resistant mutation on gyrA were detected in antibiotic-resistant strains. Mutations on rdxA were detected in both metronidazole-resistant and-sensitive strains, whereas frxA mutations were detected in only one metronidazole-sensitive strain. Finally, a rifamycin-resistant mutation in rpoB was also detected. This study provides insights into the genome of multidrug-resistant H. pylori strains of a novel sequence type circulating in Vietnam for future investigations of its pathobiology and spread through human populations.
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